Research On Correlation Between UTF1 Gene Polymorphism And Male Oligospermia And Non-Obstructive Azoospermia

Objective To investigate the correlation between UTF1 gene single nucleotide polymorphism and male oligozoospermia and non-obstructive azoospermia,and to provide new ideas for the diagnosis and treatment of male infertility.Methods 105 cases of male infertility patients from Ningxia Medical University General Hospital and Yinchuan Maternal & Child Health Hospital were selected as case groups from January 2016 to July 2018,30 healthy men who had been selected as normal control group in the same time.Taking the peripheral venous blood,UTF1 gene was sequenced by Sanger method,and the obtained sequence was compared with the database and combined with artificial reading.Finally,we selected six knowned SNPs corresponding to the database,which were rs2998111,rs3008362,rs7092433,rs11599284,rs185683664 and rs543121610.The distribution characteristics of genotype variation frequencies among the three groups were analyzed.Results There was no significant difference in age,semen volume and semen PH between the normal group,the oligospermia group and non-obstructive azoospermia group(P>0.05).The sperm concentration was significantly different among the three groups in statistics(P<0.05).The final SNPs were screened and the positive rates of genotype variation were compared among the three groups,the analysis found that only rs11599284(217G>A)sites were different among the three groups and were statistically significant(P<0.05).Correlation analysis showed that the frequency of rs11599284(217G>A)gene variation was negatively correlated with sperm concentration(R=-0.255,P<0.05).Conclusion SNP changes in the UTF1 gene rs11599284(217G>A)are associated with male oligospermia and non-obstructive azoospermia,and the variation frequency of the site is associated with decreased sperm concentration.