Analysis Of Peripheral Blood Mononuclear Cell Subtypes In Patients With Polycystic Ovary Syndrome

Objective:Detection and analysis the changes in peripheral blood mononuclear cell subsets in patients with polycystic ovary syndrome.Methods:14 patients with polycystic ovary syndrome who were admitted to the outpatient clinic of Shengjing Hospital affiliated to China Medical University from June2018 to January 2019,and 15 healthy subjects in our hospital were selected.Flow cytometry was used to detect the subtypes of peripheral blood mononuclear cells.While at the sanme time,we detect the monocyte count,lipid composition,fasting insulin,testosterone,free androgen index（FAI）,anti-cluster hormone（AMH）and other indicators,analysis of the differences between the two groups.Result:（1）The non-classical（CD14+CD16++）monocyte subpopulations were distributed between the two groups,the difference was statistically significant（p=0.005）,and the non-classical（CD14+CD16++）monocyte subsets in the polycystic ovary syndrome group were significantly elevated when compared with the control group.（2）There was no significant difference in mononuclear cell count,intermediate（CD14++CD16+）monocyte subsets and classical（CD14++CD16-）monocyte subsets between the two groups（p>0.05）.（3）The fasting insulin,free androgen index and anti-cluster hormone levels in the polycystic ovary syndrome group were significantly higher than those in the control group(p _Fins=0.018,p _PAI=0.000,p _AMH=0.004)and there was no significant difference in blood lipid composition and testosterone levels between the two groups.（4）There was a significant correlation between the non-classical monocyte subsets and the free androgen index,fasting insulin,and anti-cluster hormone AMH.Conclusion:The number of monocyte subsets associated with proinflammatory effects is significantly increased in patients with polycystic ovary syndrome.