The Safety Controlling Investigation Of Heavy Metal Polluted Xiao Chaihu Tang Preparation Based On The Establishement Of Real-time Monitoring Technology, High-efficiency Removal Material And Health Risk Assessment

An anodic square wave anodic stripping voltammetry technique based on the stable Bismuth Film electrodes material was developed for the fast screening of Cd,Pb and Cu in Xiaochaihu Tang preparation.An environmental-friendly and effective biomimetic material was prepared and applied in removing heavy metals in components preparation.In addition,Inductively Coupled Plasma Mass Spectrometry for the detection of heavy metals and harmful elements had been established in the complex matrix of traditional Chinese medicines.This confirmed the accuracy of electrochemical rapid detection technology and the removal rate of biomimetic materials.Finally,the thesis conducted a health risk assessment study on pollution levels in conjunction with relevant international and domestic standards.The main research contents and conclusions were:1.A nano-Bismuth Film electrodes material was synthesized by nano-electroplating technique,which exhibited satisfactory sensitivity and repeatability for sensing heavy metals at trace levels.And the electrochemical window was completely suitable for the detection of Cd,Pb and Cu.Combined with scanning electron microscope results,the deposition morphology of the heavy metal in the Chinese medicine matrix on the surface of the electrode material had small differences and high mechanical strength.Three kinds of electrochemical techniques（Square Wave Anodic Stripping Voltammetry,Cyclic Voltammetry,Amperometric i-t curve method）were used to compare Cd,Pb,and Cu of the oxidation-reduction peak potentials,the peak current intensity and peak shape symmetry to optimize the best detection technology and corresponding parameters.2.An anodic square wave anodic stripping voltammetry technique based on the stable Bismuth Film Electrodes material was developed for the fast screening of heavy metals.The contents of Cd²⁺,Pb²⁺,and Cu²⁺were detected to the XiaochaihuTang and and the results were consistent with those of ICP-MS.3.An environmental-friendly and effective biomimetic material was prepared and applied in removing heavy metals in Xiaochaihu Tang components preparation.The morphologies change was characterized by scanning electron microscope,transmission electron microscope,fourier transform infrared spectrometer and X-ray diffraction before and after application with biomimetic material,the pharmacodynamic influence through comparing the peak area changes in high performance liquid chromatography spectrum,the removal efficiency was tested based on the data of ICP-MS.And,the HPLC specific chromatogram of Xiaochaihu components preparation was established,baicalin was selected as the effective component to evaluate.Thus,the biomimetic material could efficiently remove heavy metals of Cd,Pb and Cu in different Xiaochaihu preparations while the loss rates of effective component of was very low.It can provide a theoretical and technical support for removing heavy metals in traditional Chinese medicine,decoctions and granules,and for further quality and safety control in any other kind of component preparations.4.Established a risk assessment model for heavy metals in traditional Chinese medicine.Transfer rate of different toxic elements before and after Xiaochaihutang compound preparation was analyzed,based on the establishment of ICP-MS heavy metals detection and analysis platform.Based on the Exposure Daily Intakes and Target Hazard Quotients,the hazard levels of heavy metals in the body of Xiaochaihu compound were evaluated.Through the analysis of health risk assessment data,the risk of heavy metals caused by the preparation of Xiaochaihu granules after preparation was smaller than that of decoction.