Study On The Mechanism Of Anti-atherosclerosis Effect Of Ginseng And Salvia Miltiorrhiza Effective Components Compatibility

Objective:Cardiovascular disease（CVD）is one of the diseases with high incidence rate and high mortality in the world.Atherosclerosis（as）is an important pathological basis of CVD.Ginseng and Salvia miltiorrhiza is one of the commonly used medicine pairs in the treatment of CVD in traditional Chinese medicine.Clinical and animal experimental studies show that the combination of them has a good effect on the prevention and treatment of as.In order to better explain the connotation and mechanism of the compatibility of ginseng and Salvia miltiorrhiza,this study intends to start with the compatibility of ginseng and Salvia miltiorrhiza group,establish an in vitro model of as,and screen the effective components of anti as of ginseng and Salvia miltiorrhiza;on the other hand,carry out qualitative and quantitative analysis of the chemical components of the effective components to network pharmacology and predict the mechanism of action.Methods:Methods:the main chemical components of Radix Salviae Miltiorrhizae and Radix Salviae Miltiorrhizae extracted by ethanol and water were analyzed qualitatively and quantitatively by HPLC,and the human umbilical vein endothelial cells（HUVECs）induced by hydrogen peroxide（H₂O₂）were analyzed by MTT,HUVEC)established oxidative stress injury model of cardiomyocytes in vitro to detect the cell survival rate,and predicted the anti as mechanism of Radix Salviae Miltiorrhizae based on network pharmacology.Results:The chemical components of ginsenosides in the decoction were analyzed qualitatively by HPLC,and ginsenosides:Rg₁,re,Rb₁,RC,Rb₂,Rd;salvianolic acids:Danshensu,rosmarinic acid,salvianolic acid B and salvianolic acid A;and ginsenosides Rg₁,re,Rb₁,salvianolic acid B and rosmarinic acid were analyzed quantitatively.The protective effect of shenshendan Decoction on HUVEC was evaluated by the injury model of human umbilical vein endothelial cells induced by H₂O₂ in vitro.The experimental results showed that different solvent extraction of shenshendan decoction could improve the survival rate of HUVEC cells（P<0.05 or P<0.01）,which was dose-dependent.The best compatibility was selected:ginseng:Salvia miltiorrhiza=1:1;according to the reported active ingredients of ginseng and Salvia miltiorrhiza,tcmid database was used to select the target for predicting the active ingredients of ginseng and Salvia miltiorrhiza,and FDA approved target for treating coronary heart disease was selected through the database of drug bank,OMIM,TTD,etc.Based on the enrichment analysis of KEGG pathway of the target,the"composition target disease"network of Radix Salviae Miltiorrhizae was constructed by using the software of Cytoscape.Network analysis showed that 117 components,25 target proteins and 11pathways were predicted to be related to coronary heart disease.GO analyzed 10 items,including monocyte differentiation,reactive oxygen species biosynthesis,regulation of precursors metabolites and energy production,and regulation of macrophage derived foam cell differentiation.There are 11 KEGG signaling pathways,including steroid biosynthesis signaling pathway,retinol metabolism signaling pathway,Fc epsilon RI signaling pathway,adipocyte factor signaling pathway,cytochrome P450 metabolism signaling pathway for allogenein and ovarian steroid production signaling pathway.It is suggested that endothelial cells,inflammatory cells and platelets are the main targets of these effective components.Conclusion:To sum up,this paper starts from the distribution of ginseng and Salvia miltiorrhiza group,establishes the in vitro model of as,on this basis,selects the effective components of ginseng and Salvia miltiorrhiza anti as;on the other hand,carries on the qualitative and quantitative analysis of the chemical components of the effective components to predict the mechanism of action by network pharmacology.To some extent,the above results revealed the connotation of compatibility of ginseng and Salvia miltiorrhiza,which laid a foundation for their rational clinical application.