The Role And Mechanism Of Hsa-miR-181b-5p In The Pathogenesis Of Acute Stanford Type A Aortic Dissection

Objective: Based on the analysis of gene chip detection results of aortic wall mesomembrane in the case-control study of early acute Stanford Atype aortic dissection,hsa-miR-181b-5p,which has significant difference in miRNA differential expression profile and has research value,was selected to explore its possible molecular mechanism in the pathogenesis of acute Stanford A-type aortic dissection by bioinformatics analysis.Methods: From January 2018 to March2019,we picked patients with acute Stanford A aortic dissection from Chinese Academy of Medical Sciences Fuwai Hospital of Shenzhen(ShenZhen Sun Yat-sen Cardiovascular Hospital).10 cases of the medial tissue of the vascular wall at the rupture of the ascending aortic dissection,10 cases of the medial tissue of the relative healthy aortic wall at the distal end of the same patient,another 5 cases of aortic wall tissue were selected for heart transplantation or aortic valve replacement at the same time.In the early stage,gene chip detection technology was used to obtain the expression profile of miRNAs in vascular wall.The target miRNA was selected after comprehensive analysis and drawing lessons from previous research results.The possible signaling pathway and pathogenesis of hsa-miR-181b-5p involved in acute Stanford type A aortic dissection were studied by bioinformatics analysis of online databases such as Microbase and David.Results: The results of microarray screening were analyzed among the three groups.Under the same screening criteria,A / C had 193 different miRNAs expressions,of which 48 were up-regulated and 145 were down regulated;A / B had 76 different expressions,41 were up regulated and 35 were down regulated;B / C had 36 different expressions,of which 14 were up regulated and 22 were down regulated.hsa-miR-181b-5p was differentially expressed in all three groups of samples,and it has research value.A total of 440 downstream target genes were predicted,and the key target gene TOP10(UBB、EP300、MAPK1)was analyzed.Through gene ontology analysis,we found that hsa-miR-181b-5p may play an important role in the pathogenesis of acute Stanford A aortic dissection by regulating 440 downstream target genes to participate in cell composition(52 items),molecular function(63 items),biological process(431 items).Through KEGG pathway analysis,33 related signaling pathways were found,among which hsa-miR-181b-5p may be involved in the pathogenesis of acute Stanford type A aortic dissection through PI3 K Akt signaling pathway,MAPK signaling pathway and adherens junction signaling pathway.Conclusion: In this study,we found that: 1.Hsa-mir-181b-5p may participate in many biological function regulation pathways by regulating 440 downstream target genes.2.Hsa-mir-181b-5p may participate in the PI3 K Akt signal pathway by regulating 18 target genes such as FGFR1,14 target genes such as mapk1,and 6 target genes such as ep300.3.There are miRNAs including hsa-mir-181b-5p in the middle membrane of aortic wall in patients with acute Stanford type A aortic dissection,which may be related to the occurrence and development of aortic dissection.