| Seleno-chitosan is a derivative of chitosan.In the present study,we sought to investigate the underlying antitumor mechanism of seleno-chitosan on human gastric cancer BGC-823 cells in vitro.MTT assay suggested that seleno-chitosan exhibited a dose-dependent inhibitory effect on the proliferation of BGC-823 cells.We found the seleno-chitosan-treated cells showed typical morphological characteristics of apoptosis in a dose dependent manner by observing on scanning electron microscope and inverted light microscope.Annexin V-FITC/PI double staining and cell cycle assay identified that the seleno-chitosan could induce BGC-823 cells apoptosis by triggering G2/M phase arrest.Our research provided the first evidence that seleno-chitosan induced the apoptosis of BGC-823 cells via an intrinsic mitochondrial pathway,as indicated by inducing the disruption of mitochondrial membrane potential(MMP),improving the levels of ROS,an increase in Bax/Bcl-2 ratio,activation of Caspase-3,Caspase-9 and Cyt-C in BGC-823 cells.The total protein maps were observed between normal BGC-823 cells and seleno-chitosan-treated cells by two-dimensional gel electrophoresis technology.11 protein spots expressed differentially and 4 were down regulated and 7 were up-regulated.5 protein spots were successfully analysed by MALDI-TOF/TOF-MS.The 5 proteins were associated with mitochondrial pathway.These combined results clearly indicated that seleno-chitosan induced apoptosis by the involvement of mitochondriamediated signaling pathway and provided precise experimental evidence for the use of seleno-chitosan as a potential agent in the prevention and treatment of human gastric cancer. |
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