Analysis Of Intestinal Flora In Patients With Hepatocellular Carcinoma And The Effect Of Different Flora On Cytokine Concentration

Objective:1.To investigate the changes in the intestinal flora of patients with primary liver cancer compared with healthy volunteers and the possible impact of different bacterial flora on the metabolic function.2.In order to detect the clinical indicators of liver function,the concentration of IL-6,IL-10 and IL-12 in patients with liver cancer.Meanwhile,analyze their correlation with different bacteria.Method:1.Collected the stool samples of 64 patients with primary liver cancer as the experimental group,and the stool samples of 24 healthy volunteers as the control group.Using the 16 SrDNA amplicon sequencing technology to perform bacterial analysis and functional prediction on the two groups of stool samples.2.We use the full-automatic biochemical analyzer to detect the liver function-related clinical indicators of the serum samples of the subjects,detect the IL-6 concentration in the serum of the subjects by the up-conversion luminescence method and detect the IL-10 and IL-12 in the serum of the subjects by ELISA.3.We use the software IBM SPSS Statistics 23 to analysis of the data.Result:1.The 16 SrDNA amplicon sequencing detected that the intestinal flora of liver cancer patients in this experiment changed compared with the intestinal flora of healthy volunteers.Bacteria are divided into seven units according to taxonomy which includes phylum,class,order,family,genus and species.There were 10,17,27,49,92,and 69 different colonies,of which the number of colonies with statistical differences(p <0.05)was 5,6,10,15,23,and 19.2.Using bioinformatics to predict the metabolic function of community samples.The control group encoded some metabolic functions(nucleotide metabolism,amino acid metabolism,lipid metabolism,energy metabolism and carbohydrate metabolism,etc.)as well as transmembrane transport,replication,translation and other functions of gene abundance was significantly higher than the experimental group.3.The clinical indicators of liver function-related parts of the experimental group are significantly different from those of the control group.The concentrations of IL-6 and IL-10 in the serum of the experimental group was significantly higher than that of the control group,and the difference was statistically significant(p <0.05),while IL-12 was the opposite.However this difference is not statistically significant(p = 0.123).4.The clinical indicators related to function,IL-6,IL-10 and IL-12 showed significant correlation with some of the different bacteria.At the genus level,there are 15 types of bacteria that show a significant correlation with liver function indexes and IL-6 and IL-10(|r|> 0.3,p <0.05),and no bacteria show a significant correlation with IL-12.However,at the species level,a total of 12 colonies showed significant correlation with liver function indicators and IL-6,IL-10,IL-12.Conclusion:1.The gut microbiota of patients with primary liver cancer and healthy volunteers showed significant differences.2.In the group of patients with liver cancer,clinical variables related to liver function showed significant differences compared with healthy volunteers.The concentrations of IL-6 and IL-10 were significantly higher than those of the control group.The clinical variables related to liver function,IL-6 and IL-10 showed strong correlation with some of the different bacteria.