| Background and objective Osteoporosis is a common and frequently occurring disease.It is characterized by decreased bone mass and degradation of bone microstructure.Its clinical manifestations are pain,increased bone fragility and fracture.WHO has listed it as the second major disease affecting public health.With the advent of aging society,the incidence rate of osteoporosis is also increasing year by year.Therefore,the research on prevention and treatment of osteoporosis has become an urgent medical topic.In recent years,many animal models have been used to study osteoporosis,including a new biological model zebrafish.Zebrafish has the advantages of small size,in vitro fertilization,short development cycle and high homology with human genes.At present,the evaluation method of bone based on zebrafish research is still mainly based on pathology,which leads to the slow progress of related research.It is urgent to explore a kind of in vivo imaging method comparable to pathology technology.Optical coherence tomography(OCT)is a kind of optical imaging technology,which uses the principle of low coherence interference and confocal scanning mode.It can image biological tissues with high resolution,and has large imaging depth as well as non-destructive imaging ability.It is expected to solve the problem of lack of in vivo imaging tools in zebrafish bone related research.The feasibility of OCT technology in evaluating the osteoporosis model of adult zebrafish in vivo was evaluated in this study.Methods First,a laboratory zebrafish breeding system was established.The second-generation juveniles produced by the wild-type zebrafish broodstock of the German AB strain were raised to 3 months old,and the second-generation adult zebrafish with normal development were selected as the experimental objects.Then,prednisolone solution was used to induce adult zebrafish to cause osteoporosis,and the establishment of zebrafish osteoporosis model was verified by processing and analyzing two types of images using pathological experimental technology and professional image processing software whether it is successful,at the same time,the OCT imaging technology is used to monitor the zebrafish model for a long time to discuss the OCT performance of the 2D and 3D images of the osteoporosis model.Results The adult zebrafish(90 days old)developed osteoporosis after being immersed in prednisolone solution for 21 days.The results of OCT imaging of zebrafish in the normal group showed that the light scattering signal of skull tissue was evenly distributed,and the photon amount remained unchanged;in the model group,the light scattering signal of skull tissue was weakened,the photon amount was significantly reduced,and the distribution was extremely uneven.The results and data analysis showed that the light density of the spinal bone of the normal group of zebrafish is about 180,the light density value of the normal group of spinal bone is about 90,the light density value of the normal group of spinal bone is about 2 times that of the model group,indicating that the adult zebrafish has had calcium loss after the ponissonina solution treatment,bone mineralization,bone density reduction phenomenon;The uneven coloring of the skull struck by the model group showed that the bone brittleness of the skull increased and the characteristics of defect fracture were confirmed,which confirmed that the zebrafish had osteoporosis.After image comparison,we found that OCT image was consistent with alizarin red staining,and the incidence of osteoporosis was positively correlated with the concentration of prednisolone solution and the length of immersion time.Conclusion This study confirmed that OCT can evaluate the osteoporosis model of adult zebrafish induced by prednisolone solution for a long time in vivo with high resolution.This further suggests that OCT can be used as an accurate,non-invasive and real-time imaging technology for the study of zebrafish osteoporosis. |
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