Association Between HSPA1L Gene Polymorphism And Preeclampsia Genetic Susceptibility

Objective In recent years,the incidence of preeclampsia has been increasing,which seriously threatens the safety and health of mothers and infants.Multiple studies have confirmed that PE patients have high levels of heat shock protein70(HSP70)expression in serum,plasma and placental tissues.HSP70 may be related to the pathogenesis of PE.In humans,three genes encode members of the HSP70 class:HSPA1A,HSPA1 B and HSPA1L.In this study,we selected three tag sites rs2227956,rs1043618 and rs1061581 in the HSPA1L gene to investigate the association between the single nucleotide polymorphism(SNP)and the genetic susceptibility to PE,aiming to provide genetic basis for clinical diagnosis and treatment of PE.Methods929 PE patients as the case group and 1024 normotensivepregnencies as the control group were enrolled from the Affiliated Hospital of Qingdao University,Yantai Yuhuangding Hospital,Binzhou Medical University Hospitalbetween October 2016 and October 2018.The peripheral blood of all subjects was collected,and the DNA was extracted and analyzed by Taqman probe real-time fluorescence quantitative PCR technique for the genotypes and allele frequencies of the three polymorphic sites of HSPA1L gene rs2227956,rs1043618 and rs1061581.Results Statistic difference of the genotypic and allelic frequencies were found in HSPA1L rs1061581 between PE patients and controls(χ~2=29.863,P<0.001 by genotype;χ~2=27.298,P<0.001,OR=1.874,95%CI 1.476-2.379 by allele).The genotypes and allele frequencies of HSPA1L rs2227956 and HSPA1L rs1043618 were not statistically different.There were significant differences in HSPA1L rs1061581 genotype and allele frequency between early-onset and late-onset PE and the control group,howeverthe HSPA1L rs2227956 and rs1043618 genotypes and allele frequencies were not significantly different.There were significant differences in HSPA1L rs1061581 genotype and allele frequency between mild / severe PE and control group,however the HSPA1L rs2227956 and rs1043618 genotypes and allele frequencies were not statistically different.Conclusions This study indicates that the HSPA1L rs1061581 polymorphism may be related to the genetic susceptibility of PE and the severity of PE in Han women.The polymorphisms of HSPA1L rs2227956 and HSPA1L rs1043618 were not associated with the genetic susceptibility of PE.