Clinical Significance Of Peripheral Blood T Lymphocytes Subsets And Nature Killer Cell Activities In The Patients With Colorectal Carcinoma

Objective:The study aimed to analysis the peripheral blood T cell subsets(CD3+?CD4+? CD8+)and NK cell of colorectal cancer patients,investigating the immune function of those patients and Its Clinical significance.Methods:In the study groups: From October 2018 to March 2019,36 patients with colorectal cancer,24 males and 12 females,aged 28-81 years,with an average age of 62.77�10.46 years,were selected.In the control group,36 healthy persons were selected from the physical examination department of the Affiliated Hospital of Chengde Medical College,including 19 males and 17 females,aged 28-79 years,with an average age of 59.67�11.96 years.Peripheral blood from two groups was obtained,and CD3+,CD4+?CD8+T lymphocyte and NK cell were analyzed by flow cytometry.The difference of immune function between healthy subjects and patients with colorectal cancer was compared and analyzed,and the relationship between T lymphocyte subsets,NK cell expression and clinicopathological features of patients with colorectal cancer was judged.Results:1.The percentage of CD3+,CD4+T lymphocyte,NK cells and CD4+/CD8+ ratio in peripheral blood of patients with colorectal cancer was respectively(72.4�11.11;43.40�9.65;2.07�0.97;13.21�9.26)%.Each of them was obviously higher than controls(76.91 � 6.82;47.58 � 5.40;2.90 �0.61;19.94�4.07)%,with statistical significance(P<0.05).2.There was not statistical significance between the percentage of T lymphocyte subsets and NK cells in peripheral blood of patients with differentage,sex,KPS score,NRS2002 score.(P > 0.05).3.T lymphocyte subsets and NK cells in peripheral blood of patients with colorectal cancer were not related to tumor location and differentiation(P > 0.05),but to TNM stage and lymph node metastasis(P < 0.05).The percentage of CD4+ T cells and CD4+/CD8+ ratio in peripheral blood of of patients with cancer at I+II stage was respectively(46.96�9.28;2.53�1.07)%,while III+IV stage was(39.85�8.88;1.61�0.61)%,there was statistical significance between different stage(P<0.05),which mean that the percentage of CD4+ T cells and CD4+/CD8+ ratio was higher in the early stage than the last.The percentage of CD8+T cells in peripheral blood of patients at III+IV stage was(26.57 �6.03)%,which was obviously higher than I+II stage(21.33 � 8.25)%,with statistical significance(P<0.05).The percentage of CD4+T cells and CD4+/CD8+ ratio in peripheral blood of patients with lymph node metastasis was(38.54�8.50;1.56�0.59)% respectively,which were lower than without lymph node metastasis(46.87 � 9.06;2.44 � 1.04)%.There was statistical significance between different group(P<0.05).The expression of NK cells was related to the depth of tumor invasion(P< 0.05),but not to TNM stage and lymph node metastasis(P> 0.05)Conclusion:1.Detection of T lymphocyte subsets and NK cells in peripheral blood of patients with colorectal cancer is helpful to evaluate the immune function of patients.Compared with healthy people,colorectal cancer patients have lower immune function.2.There was statistical significance between the percentage of CD4+/CD8+ ratio in peripheral blood of patients with TNM stage and lymphatic metastasis.The smaller ratio,the later the stage,the worse the prognosis.3.Detection of immune function of peripheral blood cells in patients with colorectal cancer is helpful to judge the disease condition,screen high risk groups,and provide help for early clinical diagnosis and prognosis.