| ObjectiveTo investigate the effect of CYP4F2 expression on the proliferation,metastasis and apoptosis of hepatocellular carcinoma cells in vitro,and to explore its mechanism of action.MethodsHuman normal liver cells LO2 and human hepatocellular carcinoma cells Hep3B were cultured in vitro,and the expression levels of CYP4F2 were detected by QT-PCR and western blotting,and the differences were compared.Then we transfected Hep3B cells with lentivirus to make it overexpress CYP4F2,and the expression of CYP4F2 was verified by QT-PCR and western blotting.The proliferation,migration and apoptosis levels of liver cancer cells before and after transfection were detected and compared by CCK8 cell proliferation experiment,Transwell cell migration experiment,wound healing assay,and flow cytometry analysis.Finally,the changes in the Nrf2 signaling pathway before and after transfection were detected by westem blotting.ResultsCompared with normal human liver cancer cell LO2,the expression level of CYP4F2 gene in human liver cancer cell Hep3B was significantly reduced The expression level of CYP4F2 was significantly increased after lentivirus transfection,and the proliferation and migration of liver cancer cells were significantly inhibited after transfection.In addition,CYP4F2 overexpression inhibited the expression of Nrf2 signaling pathway members(such as Nrf2,HO-1 and FTH1)The increased expression of NQO1 indicated that the overexpression of CYP4F2 reversed the anti-oxidative response of Hep3B cell,and the increased expression of NQO1 may also be one of the reasons for its inhibition of Hep3B hepatocellular carcinoma proliferation and promotion its apoptosis.ConclusionCYP4F2 expression were considerably lower in Hep3B hepatoma cells relative to LO2 cells.CYP4F2 inhibits the proliferation and migration of liver cancer cells through Nrf2/HO-1 signaling,and the increased expression level of NQO1 may also be involved in inhibiting the spread of Hep3B liver cancer and promoting its development. |
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