MMP2 And MMP10 Polymorphisms Are Related To Steroid-induced Osteonecrosis Of The Femoral Head Among Chinese Han Population

Background:Steroid-induced osteonecrosis of the femoral head is a relatively serious condition which seriously reduces patient quality of life.However,the pathogenesis of steroid-induced ONFH is still unclear.In recent years,more scholars have found that the pathogenesis of steroid-induced ONFH is related to susceptibility factors such as MMPs/TIMPs system.The main purpose of this study is to investigate the correlation between MMP2 and MMP10 gene polymorphisms and steroid-induced ONFH in Chinese Han population.Methods: Six SNPs in MMP2 and two SNPs in MMP10 were genotyped using Agena Mass ARRAY RS1000 system from 286 patients of steroid-induced ONFH and in 309 healthy controls.The association between MMP2 and MMP10 polymorphisms and steroid-induced ONFH risk were estimated by the Chi-squared test,genetic model analysis,haplotype analysis,and stratification analysis.The relative risk was estimated by odd ratios(ORs)and 95% confidence intervals(CIs).Result: We found that the minor TG allele of rs470154 in MMP10 was associated with an increased risk of steroid-induced ONFH(OR = 1.45,95% CI,1.03 – 2.05,p = 0.032).In the genetic model analysis,we found that rs2241146 in MMP2 gene and rs470154 in MMP10 gene showed a statistically significant association with increased risk of steroid-induced ONFH.The six SNPs(rs470154,rs243866,rs243864,rs865094,rs11646643,and rs2241146)showed a statistically significant association with different clinical phenotypes.Conclusion: Our results verify that genetic polymorphisms of MMP2 and MMP10 contribute to steroid-induced ONFH susceptibility in the population of Chinese Han population,and our study provides new insights into the role that MMP2 and MMP10 plays in the mechanism of ONFH.