Secondary Metabolites From The Mangrove Endophytic Fungus,Phomopsis Sp.xy21

Mangrove forests constitute a dynamic transition zon between terrestrial and marine habitats and mangrove plants have to adapt to extreme environmental conditions,including extensive salinity,high temperature and moisture,anaerobic soil,tidal activities and high microbial and faunal competition.Hence,endophytes from manrove plants have recently attracted considerable attention from natural products looking for new inspirations from nature.In this paper,we focus on searching for bioactive secondary metabolites from the mangrove endophytic fungus Phomopsis sp.21.Phomopsis.sp 21 was isolated from the leaves of Xylocarpus granatum collected from the Thailand,which was chemically investigated by means of repeated gel chromatoraphies,incuding silica gel,ODS(C₁₈)column,and Sephadex LH-20chromatographies,as well as semi-preparative HPLC.This work led to the isolation and identification of 31 compounds.The structures of all compounds were indentified by NMR,HRESIMS,UV,X-ray diffraction.Among them there were 1 polyketone?1?,1 chromone?2?,6xanthones?3-6,14-15?,9 dimeric xanthones?8-9,20-26?,3 flavonoids?7,16-17?,5 lactones?10,19,27-29?,3 azaphilones?11-13?,1 lipopeptides?30?,1 cytochalasan?31?.Compounds?1-11?are new ones.Anti-tumor and anti-HIV activity of some compounds were evaluated.Compound2,9,22,24 show a certain cytotoxic activity against C6 glioma cell lines and compound 3 show a weak inhibition on HIV virus.