A Study Of The Relationship Between 15 Lipid Metabolism-Related Gene Loci Polymorphisms And Leucocyte Telomere Length In Elder Adults

Objective: Telomere length is one of the biomarkers of cellular aging,which associated with mental disorders,dementia and some kind of cancer closely.Howere,the determinants of telomere length still unclear.This study aims to explore the relationship between lipid metabolism related genetic loci polymorphisms and leukocyte telomere length(LTL),and to exam the potential determinants of cellular aging,and provide evidence for the early identification of and precision prevention of aged-related diseases.Methods: Based on a cross-sectional study enrolled of 2,785 community residents who participated in the regular public health check in Yinchuan and Wuzhong of Ningxia province from 2013 to 2016.Face-to-face interview conducted to collect general information during the health check;matrix-assisted laser desorption electro-time of flight mass spectrometry was used to categories the polymorphisms of 17 lipid metabolism related loci;and quantitative real time polymerase chain reaction(qPCR)was used to measure the LTL.General linear regression model employed to exam the association between genes and LTL.Results: 1.A total of 1734 subjects with the data of LTL,genotypes include in the final analysis,of them,811(46.77%)are males and 923(53.23%)are females,the average age is 63.63±4.89 years;The univariate analysis showed that age inversely associated with the LTL(t = 2.40,P = 0.016);the LTL in the non-smoker group was greater than that in the smoking group(t= 2.24,P= 0.025),and there were statistically significant differences in LTL among different rs4402960 genotypes(F= 7.29,P=0.001).2.The general linear regression showed that in the dominant model,age andsmoking were negatively correlated with LTL(B =-0.01,P = 0.001;B =-0.17,P =0.032,respectively);In the recessive model,the rs2075650 was positively correlated with LTL,while age,rs4402960 and rs8181588 were negatively correlated with LTL(B = 0.56,P = 0.022;B =-0.01,P = 0.002;B =-0.12,P = 0.001;B =-0.21,P = 0.010,respectively);In the codominant model,age,rs4402960 and rs8181588 were negatively correlated with LTL(B =-0.01,P = 0.001;B =-0.09,P = 0.003;B =-0.10,P = 0.035,respectively).3.When the model stratified by gender,general linear regression analysis found that in men,age(B =-0.01,P = 0.027),smoking(B =-0.16,P = 0.047),rs8181588(B=-0.33,P = 0.007)and rs2075650(B = 0.85,P = 0.027)were associated with LTL in recessive genetic model;and age(B =-0.01,P = 0.024),smoking(B =-0.17,P =0.035)and rs8181588(B =-0.15,P = 0.029)were associated with LTL in codominant genetic model.Among women,age(B =-0.01,P = 0.027)and rs4402960(B =-0.19,P <0.001)were associated with LTL in recessive genetic model;and age(B =-0.01,P= 0.016),rs4402960(B =-0.13,P =0.001)were associated with LTL in codominant genetic model.4.When stratified by age group,general linear regression analysis found that for subjects aged under 65 year,rs4402960(B =-0.16,P = 0.001)was associated with LTL in recessive genetic model;and rs4402960(B =-0.12,P = 0.002)was associated with LTL in codominant genetic model.For subjects 65 years and older,rs2283228(B= 0.30,P = 0.017),rs7901695(B = 1.69,P = 0.002)and rs8181588(B =-0.38,P =0.002)were associated with LTL in recessive genetic model;and rs2075650(B = 0.23,P = 0.007),rs2283228(B = 0.17,P = 0.023)and rs8181588(B =-0.19,P = 0.010)were associated with LTL in codominant genetic model.Conclusions: 1.Age and smoking are important environmental factors that affect the telomere length of peripheral blood granulocytes;2.Inherited genetic factors have a certain effect on the telomere length of peripheral blood granulocytes.Gene locus such as rs8181588,rs2283228,rs4402960,rs7901695 and rs2075650 are important factors affecting the length of telomere.The above findings suggest that genetic polymorphism may be closely related to cell aging,and provide a reference for early identification of aging-related diseases and precise prevention of individuals.