| BackgroundIn recent years,the proportion of infertility in the childbearing age couples is higher and higher,and the male infertility accounts for about 40 ~ 60%.At present,there is a lack of more sensitive biomarkers to evaluate male reproductive health.During spermatogenesis,the telomere length remains unchanged to maintain the stability of sperm chromosomes.There is a correlation between telomere length and semen quality.Telomere length change may become a new and easy to measure biomarker to detect male reproductive damage.ObjectiveTo understand the current level of semen quality in men of childbearing age in Puyang City,Henan Province.Analyze the relationship between relative telomere length and semen quality,evaluate its possibility as a biomarker of male reproductive damage,and study the correlation between genetic factors and relative telomere length.MethodsThe study population came from male patients in reproductive health center of Puyang maternal and child health hospital.The effects of relative telomere length and related gene polymorphism on semen quality were analyzed by computer-aided sperm analysis,polymerase chain reaction,real-time fluorescent quantitative PCR and restriction fragment length polymorphism.Results1.Semen quality related resultsWith the increase of age,semen volume,total sperm count,percentage of forward motile sperm,total motility percentage,percentage of forward motile sperm with VSL greater than 35μm/s basically showed a downward trend;with the increase of BMI,the frequency of whipping generally showed a downward trend;the curve speed,straight-line speed,average path speed,sperm head sway amplitude of drinking group were higher than those of non drinking group.2.The effect of GSTT1 and GSTM1 gene deletion polymorphism on semen qualityBy univariate logistic regression analysis,GSTM1 gene was the protective factor for the percentage of forward motile sperm(OR=1.68,95% CI: 1.08 ~ 2.61),and GSTT1 gene was the protective factor for the percentage of total sperm motility(OR=1.75,95% CI: 1.08 ~ 2.83).By multivariate logistic regression analysis,GSTM1 gene was the protective factor for the percentage of forward motile sperm(OR=1.68,95% CI: 1.08 ~ 2.62),and GSTT1 gene was the protective factor for the percentage of total sperm motility(OR=1.75,95% CI: 1.08 ~ 2.83).3.Analysis of relative telomere length and semen qualitySpearman correlation analysis showed that relative telomere length was positively correlated with sperm count,sperm concentration,percentage of forward motile sperm and VSL,and negatively correlated with SDI and neck defect.By multiple linear regression analysis,there was a positive correlation between relative telomere length and sperm count(β=2.03(0.08,3.99),P<0.05).4.Analysis of gene polymorphism and telomere lengthBy multiple linear regression analysis,the relative telomere length of Pot1 rs1034794 genotype AT(β=-7.81(-13.40,-2.21),P<0.05)and TT(β=-9.51(-15.39,-3.63),P<0.05)was shorter than that of AA,tert rs2735940 genotype TT(β=4.92(0.04,9.79),P<0.05)was longer than that of CC,and TERC rs2293607 genotype GG(β=-4.56(-8.86,-0.26),P<0.05)was shorter than that of CC.5.Analysis of gene polymorphism and semen qualityBy univariate logistic regression analysis,genotype AT(OR=3.21,95%CI: 1.10~9.37)and TT(OR=3.90,95%CI:1.37~11.15)compared with genotype AA of POT1 rs1034794 and genotype CC(OR=5.17,95%CI: 1.44~18.53)compared with genotype AA of POT1 rs10250202 were associated with the increased percentage of forward motile sperm.Genotype CC(OR=5.05,95%CI: 1.08~23.53)compared with genotype of POT1 rs10250202 was correlated with the increase of total activity percentage.6.The protective effect of spicy food on semen qualityUnivariate binary logistic regression analysis showed that after adjusting for age,smoking,drinking and BMI,food spicy degree,spicy time,weekly spicy frequency and monthly spicy frequency were protective factors of semen quality parameters.Multivariate logistic regression analysis showed that after adjusting for age,smoking,drinking and BMI,spicy time was a protective factor for sperm count and sperm concentration.Conclusion1.With the increase of age,the basic parameters of semen decreased.With the increase of BMI,the frequency of sperm whipping decreased.2.GSTM1 gene is a protective factor for the percentage of forward motile sperm and GSTT1 gene is a protective factor for the percentage of total sperm motility.3.Genetic polymorphism may lead to the change of semen quality by changing telomere length.4.There were positive correlations between food spicy degree,spicy time,spicy frequency per week,spicy frequency per month and semen basic parameters and sperm motility parameters. |
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