Effects Of Different Ways Of Exercise On Hepatic Lipid Metabolism In Rats And Its Mechanism

Objective: Observe the change of weight,blood lipids,hepatic lipids,liver AMPK? and ACC phosphorylation levels in rats after long-term training,one-time exhaustive exercise and long-term training combined with one-time exhaustive exercise separately to compare the effects of three exercise modes on the liver lipid metabolism in rats and analyze the regulatory mechanisms of different ways of exercise on the liver lipid metabolism in rats.Methods: Forty male SD rats aged 4 weeks were randomized into four groups after a period of 1 week of adaptive feeding,respectively quiet group(Q group),long-term training group(LT group),one-time exhaustion group(OE group)and long-term training combined with one-time exhaustion group(LO group),10 in each group.The LT group was sampled the next day after fasting for 24 hours after the training.The OE group and the LO group were sampled immediately after the last exercise.COD-PAP method was used to measure rat serum and liver TC and TG.Rat serum and liver HDL-C and LDL-C levels were detected according to the kit instructions.Serum levels of FFA and liver were determined by copper chromogenic assay.The phosphorylation levels of AMPK? and ACC in the liver of the rats in four groups were detected separately by Western blot.Results:(1)Compared with those in group Q,weight of the rats in LT group and LO group were significantly decreased(P<0.05),and rats in LO group was had lower weight than those in LT group(P<0.05).Compared with those in OE group,weight of the rats in LO group significantly decreased(P<0.05).(2)Compared with those in group Q,serum TG,TC and LDL-C levels of rats in LT group significantly decreased(P<0.05),but serum FFA level of rats in LT group and serum TG and FFA level of rats in OE and LO group significantly increased(P<0.05);Compared with those in group LT,serum TG,TC and LDL-C levels of rats in OE and LO groups significantly increased(P<0.05),serum FFA level of rats in group OE significantly decreased(P<0.05),but serum FFA level of rats in group LO significantly increased(P<0.05);Compared with those in OE group,serum TG and LDL-C level of rats in LO group significantly decreased(P<0.05),but serum FFA level of rats significantly increased(P<0.05).(3)Compared with those in group Q,liver LDL-C level of rats in LT group,OE group and LO group significantly increased(P<0.05),liver TG level of rats in LT group significantly decreased(P<0.05),and liver FFA level of rats in LT group significantly increased(P< 0.05);liver FFA level of rats in OE group significantly decreased(P<0.05),but liver FFA level of rats in LO group significantly increased(P<0.05).Compared with those in LT group,liver TG level of rats in OE group and LO group significantly increased(P<0.05).Compared with those in LT group,the liver FFA level of rats in OE group significantly decreased(P<0.05),but the liver FFA level of rats in LO group significantly increased(P<0.05);Compared with those in OE group,the liver FFA level of rats in LO group significantly increased(P<0.05).(4)Compared with those in Q group,phosphorylation level of hepatic liver AMPK? of rats in OE group significantly decreased(P<0.05),but phosphorylation level of hepatic liver AMPK?of rats in LO group significantly increased(P<0.05);Compared with those in group LT,the phosphorylation level of AMPK? of rats in group OE significantly decreased(P<0.05);The phosphorylation level of liver AMPK? of rats in LO group significantly increased(P<0.05);Compared with those in group OE,the phosphorylation level of liver AMPK? of rats in group LO significantly increased(P<0.05).(5)Compared with those in group Q,the phosphorylation level of liver ACC of rats in group LT,group OE and group LO significantly increased(P<0.05);Compared with those in group LT,the phosphorylation level of liver ACC of rats in group OE significantly decreased(P<0.05)and The phosphorylation level of liver ACC of rats in group LO significantly increased(P<0.05);Compared with those in group OE,the phosphorylation level of liver ACC of rats in group LO significantly increased(P<0.05).(6)Liver TG was positively correlated with liver TC(r=0.744),liver TG was negatively correlated with serum TG(r=-0.456),liver TG was positively correlated with P-AMPK(r=0.386).There was a positive correlation between serum LDL-C and serum TC(r=0.699).There was a positive correlation between liver FFA and liver HDL-C(r=0.506);serum FFA was positively correlated with liver FFA(r=0.419);serum FFA was positively correlated with P-AMPK(r=0.528);serum FFA and P-ACC were positively correlated(r=0.751).There is a positive correlation between P-AMPK and P-ACC(r=0.513).Conclusions:(1)After one-off exhaustive exercise,the rat serum indexes,namely both FFA and TG increase,while the rat liver FFA declines.When the ACC phosphorylation level goes up,the AMPK? phosphorylation level goes down.All of them indicate,the overall adjusting effect upon fat mobilization is predominated by ACC.(2)Through a long-term training,the rat serum indexes,inclusive of TG,TC and LDL-C stay in drastic declines,while the FFA levels of rat liver and serum constantly increase along with the upward trend of AMPK? phosphorylation level,which gives the better clue that long-term training can aid in breaking down fats and lowering blood lipids by adjusting up ACC phosphorylation process.(3)With combination of along-term training and one-off exhaustive exercise,the FFA levels of rat serum and liver are all on-the-increase,accompanying with the upward trend of both phosphorylation levels ofAMPK? and ACC,which indicates integrating long-term training with one-off exhaustive exercise can activate signalling pathway of AMPK#ACC and improve entire lipid metabolism.