Ginsenoside Rh1 Inhibits Breast Cancer Proliferation And Metastasis

Purpose:To observe the effects of ginsenoside Rh1 on proliferation and invasion of human breast cancer cells MDA-MB-231 and MCF-7,and to explore the possible mechanism.To investigate the effect of ginsenoside Rh1 on the expression of three genes of DDIT3 and HYAL1 and PLD1 in human breast cancer cells,MDA-MB-231 and MCF-7,and to verify the inhibitory effect of ginsenoside Rh1 on breast cancer and to explore possible targets.Material and method:Ginsenoside Rh1 acts on triple negative breast cancer cell MDA-MB-231 and estrogen receptor positive human breast cancer cell MCF-7 respectively.The inhibitory effect is observed and the inhibitory rate is calculated.The semi inhibitory concentration(IC₅₀)is obtained by the Origin software.The optimum concentration of the drug on the cell is calculated according to the IC50,and the MTT method is used.Detect cell proliferation ability,observe cell invasiveness through Transwell chamber,observe cell activity and proliferation and metastasis ability of stem cells,detect the expression of DDIT3and HYAL1 and PLD1 in cells by RT-PCR method,and explore whether the differential expression of these genes in breast cancer cells can regulate the proliferation and metastasis of breast cancer,thus we can find the possible target of ginsenoside Rh1 in breast cancer.Results:1.It was found that ginsenoside Rh1 had inhibitory effect on MDA-MB-231 and MCF-7 of human breast cancer cells.The inhibitory effect of ginsenoside Rh1 on human breast cancer cell MDA-MB-231 was stronger,the maximum inhibition rate was 82.66%,IC₅₀ was between0.59-0.64 x 10^-3mol/L,and the maximum inhibition rate of MCF-7 cells was 80.46%,IC₅₀ in0.63-0.71 x 10^-3mol/L.The larger the value of IC₅₀ showed weak inhibitory effect.2.When the concentration of ginsenoside Rh1 was 250μM,500μM and 750μM,the maximum inhibitory rates of MDA-MB-231/MCF-7 in human breast cancer cells were23.31%/18.93%,43.18%/39.53%,82.45%/80.39%.The difference was statistically significant when the concentration of ginsenoside Rh1 concentration was 750 micronμM,and the difference was statistically significant（P<0.05）,and with the prolongation of drug action time,the inhibitory effect also increased,indicating that ginsenoside Rh1 has obvious concentration dependence and time dependence.When the concentration of ginsenoside Rh1 was 750μM,the cell growth curve was drawn.The same concentration of ginsenoside Rh1 inhibited the proliferation of MDA-MB-231 cells stronger than that of MCF-7 cells,and the difference was statistically significant（P<0.05）.3.We count the number of cells passing through the basement membrane for 24 hours,the results showed:MDA-MB-231 experimental group（21+1.14）,MDA-MB-231 control group（85.6+2.18）,the experimental group was significantly different from the control group（P<0.05）,MCF-7 experimental group（25.7+1.25）,MCF-7 control group（84.8+1.37）,the experimental group was compared with the control group.The difference was statistically significant（P<0.05）.Compared with the inhibition rate of ginsenoside Rh1 on MDA-MB-231cell invasion ability（75%）and the inhibition rate of MCF-7 cell invasion ability（69%）,the difference was statistically significant.4.We found that ginsenoside Rh1 had an effect on the ability of tumor cells to form the ball.After a week,the percentage of cells in the control group was significantly higher than that in the experimental group,and the difference was statistically significant（P<0.05）.Compared with the MCF-7 experimental group,the MDA-MB-231 test ball rate was lower,and the difference was statistically significant（P<0.05）.5.RT-PCR showed that ginsenoside Rh1 could differentially express DDIT3,HYAL1 and PLD1 in two kinds of cells,but only the low expression of HYAL1 was statistically significant.Conclusion:1.Ginsenoside Rh1 had inhibitory effects on human breast cancer cells MDA-MB-231 and MCF-7,among which ginsenoside Rh1 had a strong inhibitory effect on human breast cancer cells MDA-MB-231,IC₅₀ values Between 600-700μM.2.In this experiment,ginsenoside Rh1 has obvious concentration dependence and time dependence.When the concentration of ginsenoside Rh1 is 750μM,the inhibitory effect on the proliferation of MDA-MB-231 and MCF-7 in human breast cancer cells is the strongest,and the inhibition effect increases with the prolongation of drug action time.The same concentration of ginsenoside Rh1 inhibited the proliferation of MDA-MB-231 cells stronger than MCF-7 cells,the difference was statistically significant（P<0.05）.3.In this experiment,ginsenoside Rh1 reduces the vitality of human breast cancer MDA-MB-231 and MCF-7 cells,affects cell proliferation and invasion ability,inhibits the proliferation and metastasis of MDA-MB-231 and MCF-7 in breast cancer cells,and is stronger than MCF-7 cells in MDA-MB-231 cells.4.In this experiment,ginsenoside Rh1 could inhibit the formation of MDA-MB-231 and MCF-7 in breast cancer cells.It shows that ginsenoside Rh1 may have an effect on the Stem cell characteristicsof breast cancer cells.5.The gene HYAL1 may be the target of ginsenoside Rh1 inhibiting the biological behavior of breast cancer.