| Objective: using immunohistochemical technology clear water channel protein 7(AQP7)and water channel protein 10(AQP10)in recurrent abortion(RSA)rats decidual the differentially expressed and the positioning of distribution situation,from the protein molecular level research Shoutaiwan and its active ingredient treatment RSA may new mechanism and new targets,improve the level of traditional Chinese medicine for prevention and treatment of spontaneous abortion,provide new train of thought and experimental basis for prevention and control of traditional Chinese medicine(TCM)RSA.Methods: 60 female mice and 30 male mice were mated in a ratio of 2:1.Pregnant mice were observed on the first day after pregnancy.60 pregnant mice were randomly divided into6 groups: the normal control group(group A),the model group(group B),the western control group(group C),the low dose group(group D),the medium dose group(group E),and the high dose group(group F),with 10 pregnant mice in each group.A large number of spermatozoa were observed on the vaginal smear,and it was found that the female mice had vaginal plug shedding,which was used as the first day of pregnancy in pregnant mice.The pregnant mice in each group(except the normal control group)were given hydroxy urea(450mg·kg-1)dissolved in 2m L normal saline every afternoon from the 1st day of pregnancy to the 9th day of pregnancy.On the morning of the 10 th,pregnant mice were given 2m L normal saline with mifepristone(RU486)4.0mg/kg solution to create a recurrent abortion model.In addition,each group of group D(0.5 g/kg),group E,(1 g/kg),group F(2 g/kg),and group C(3.02 mg/kg)was given corresponding therapeutic drugs by intragastric administration in the morning and afternoon of each day.The model group and the normal control group were given equal volume normal saline for 9 consecutive days.On the 11 th day of gestation,the rats were anesthetized and sacrificed.The uterine decidua of the rats was isolated and sectioned after embedding.Using immunohistochemical testing AQP7 and AQP10 positioning and quantitative expression,collecting pictures after using the microscope observation AQP7,AQP10 site,expressed in cells in the at the same time use IPP Image(Image-Pro)6.0 software analysis,calculation IOD/Area measurement,IOD/Area as half quantitative statistical analysis parameters.Results:1.AQP7 and AQP10 were expressed in the decidua of SD rats,and the cell membrane and plasma were the expression sites.In the decidual of RSA rats,AQP10 expression was down-regulated,and after intervention by different factors,AQP10 expression was up-regulated in the decidual of the rats in the ditreprogesterone group and the low,medium and high dose group.Life embryo pills had no obvious regulating effect on AQP7.2.Compared with the normal pregnancy group,the expression of AQP7 in the decidual tissues of pregnant mice in the model group was low(P>0.05).Compared with the normal control group,the expression of AQP10 in the model group was significantly decreased,and the difference was statistically significant(P < 0.01),indicating that the model of kidney deficiency luteinizing inhibition was successful.The AQP10 expression was decreased in the low dose group of Shoutaiwan and the group of diquprogesterone,and the difference was statistically significant(P < 0.01).The results showed that the expression of AQP10 was significantly increased in all dose groups of Shoutaiwan and the western drug control group and the model group(P<0.01).The comparison between the low-medium dose group and the western medicine control group and the high-dose group suggested that the expression of AQP10 in the high-dose group was higher than that in the other groups,and the difference was statistically significant(P < 0.01).There was no significant difference between the medium dose group of Shoutaiwan and the group of diquprogesterone(P>0.05).Conclusion:1.The exuduvial membrane and cytoplasm of uterus of SD rats with recurrent abortion are the expression sites of AQP7 and AQP10.2.AQP10 expression was down-regulated in uterine decidua of SD rats with recurrent abortion,but AQP7 expression was not significant.3.AQP10 may be the antifoetal target of Shoutaiwan and the molecular material basis of "kidney water". |
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