| Objective:Gastrointestinal heat accumulation refers to the accumulation of intangible heat evil or tangible heat accumulation in the gastrointestinal tract,which leads to the heat accumulation of gastrointestinal gas.Previous studies have found that intestinal flora structure is disordered in the state of gastrointestinal accumulated heat,the abundance of bacteria changes to different degrees,and intestinal mucosal immune function is also affected.In terms of the physical characteristics of the gastrointestinal tract,the frequency and amplitude of electrogastrogram(EGG)increase rapidly and the gastrointestinal motor function is obviously abnormal under gastrointestinal accumulated heat.However,the research on specific physical characteristics indicators is relatively insufficient.Gastrointestinal stress,temperature and humidity are important indicators of physical characteristics of the gastrointestinal tract.In this study,the above three physical indicators will be selected and combined with chemical indicators,such as the pH of intestinal contents,gastrointestinal hormones and gastrointestinal motility proteins,to effectively compare and connect physical indicators and chemical indicators,so as to clarify the relationship between the physical characteristics of gastrointestinal tract and digestive function under the condition of gastrointestinal heat accumulation.At the same time,the biological mechanism of gastrointestinal motility changes under the condition of gastrointestinal tract heat is explored in order to better understand the mechanism of gastrointestinal tract physical characteristics affected by gastrointestinal tract heat.Method:Rats were randomly divided into the normal group,the gastrointestinal tract heat group,and the mosapili treatment group.General signs such as hair color,mental state,and feces were observed and recorded,and data such as collective mass,anal,toe,and axillary temperature were collected.They were harvested after a week of feeding.Detection of viscera index;The pathological changes of colon tissues were observed.In vitro sensors were used to detect the stress,temperature and rectal temperature in the projected area of abdomen and intestines of rats.PH meter detects the pH of cecal contents;The contents of cecum and excrement are dried in an electric thermostatic air-blast drying box and the humidity changes are calculated.The propulsive rate of small intestine was measured by semi-solid nutrient pastes.Serum NO,GAS and SP were detected by Elisa.The expression of BDNF and Cx43 were detected by immunohistochemistry and Western Blot.Rt-qpcr was used to detect BDNF mRNA and Cx43 mRNA expression in intestinal tissues of rats.Result:1.General condition:Normal Group of rats fur smooth white glossy,lively and active,clear urine,stool color brown black,heavy weight.The fur of rats in the gastrointestinal heat accumulation group was more fluffy,the color was yellow and lusterless,the whole spirit was bad,the urine was yellow,the stool was constipated,the color was dark and globular.After the drug intervention,mosapride treated group rats’mental state improved,hair color was normal,urine returned to Pale yellow,but mosapride treated group rats’stool shape was still spherical.Compared with the normal group,the body weight of the rats in the heat group decreased significantly(P<0.05),and the body weight of the mosapride group increased in the 6th to 7th day(P<0.05).There was no significant difference in anal temperature between groups(P>0.05).Compared with the normal group,the toe temperature of the rats in the heat group increased on the 3rd day of the experiment(P<0.05).There was no significant difference in the finger temperature between the rats in the mosapride group and the rats in the heat group(P>0.05).Compared with the normal group,the axillary temperature in the heat group increased on the 3rd day,and that in the mosapride group decreased on the 6th day(P<0.01).2.Organ Index:Compared with the normal group,the spleen index of the rats in the heat group was significantly decreased(P<0.05),but the lung,kidney and Thymus index were not significantly different(P>0.05).The indexes of lung,spleen,kidney and thymus of rats in Mosapride treatment group were not significantly different from those in gastrointestinal heat accumulation group(P>0.05).3.Colon histopathology:under the microscope,there were no hyperemia and inflammatory cells in the muscle layer of normal rats,there were many goblet cells arranged in order,and the single-layer columnar cells were intact.There was no obvious pathological change in the group of heat accumulation of stomach and intestine and the group of Mosapride.4.Intestinal physical indexes:(1)Stress:compared with the normal group,the stress in the abdominal intestinal projection area of the rats with heat accumulation of stomach and intestine had no significant difference(P>0.05).(2)Temperature:On the 2nd and 4th day of the experiment,the rectal temperature of the rats in the heat group was significantly higher than that in the normal group(P<0.01),and on the 3rd day,the rectal temperature of the rats in the heat group was significantly higher than that in the normal group(P<0.05).At 1-4 days,there was no significant difference in the temperature of the abdominal intestinal tract projection area between the rats with heat accumulation of stomach and intestine(P>0.05).(3)Humidity:compared with the normal group,the humidity of cecum contents decreased(P<0.05),but the fecal humidity did not change significantly(P>0.05).(4)pH:compared with the normal group,there was no significant difference in the pH of the cecum contents of rats in the group of heat accumulation of stomach and intestine(P>0.05).(5)Small intestine propulsive rate:compared with the normal group,the propulsive rate in the heat group was lower(P<0.05).5.The level of brain-gut Hormone in Serum:Compared with the normal group,the level of NO increased(P<0.5),SP decreased(P<0.5),GAS had not significant difference(P>0.5).6.The expression of Cx43,BDNF protein and mRNA:(1)immunohistochemical detection results:the expression of BDNF protein in colon tissue of rats in each group was not significantly different.Compared with the normal group,the expression of Cx43 protein in the mosapride group was decreased,and that in the mosapride group was decreased(P<0.01).(2)Western blotting results:Cx43 Index:compared with the normal group,the expression of Cx43 protein in the colon of rats in the peptic fever group and Mosapride Treatment Group was significantly decreased(P<0.01).The content of Cx43 protein in colon of rats in mosapride group was significantly higher than that in gastro intestinal heat group(P<0.01).BDNF Index:compared with the normal group,the expression of BDNF protein in Colon was significantly decreased in the heat group and mosapride group(P<0.01).The expression of BDNF protein in colon of rats in mosapride group was significantly higher than that in gastro intestinal heat group(P<0.01).(3)RT-qPCR results:Expression of Cx43 mRNA:Compared with the normal group,the Cx43 mRNA expression in the colon tissues of rats in the gastrointestinal heat group and the mosabili-treated group was significantly decreased(P<0.01).The Cx43 mRNA expression in the colon tissues of rats treated with mosabili was significantly increased(P<0.01)compared with that of the gastrointestinal heat group.Expression of BDNF mRNA:compared with the normal group,the expression of BDNF protein gene in colon tissue of rats in gastrointestinal heat storage group and mosapride group was significantly decreased(P<0.01).The expression of BDNF gene in colon tissue of rats treated with mosapride was significantly higher than that of rats treated with heat accumulation of stomach and intestine(P<0.01)Conclusion:Heat accumulation in the stomach and intestines can change the physical characteristics of the intestine.The temperature of the rectum increases,the moisture content of the cecum content decreases,while the pH and the moisture content of the feces of the cecum content decrease,the stress and temperature of the abdominal intestinal tract in rats showed a decreasing trend,but there was no significant change.Heat accumulation in the stomach and intestines can reduce the intestinal propulsion rate of the organism and thus cause gastrointestinal motility deficiency.The mechanism of gi accumulative heat may be related to the fact that GI accumulative heat inhibits the release of substance P by affecting the expression of BDNF and ultimately leads to GI motility deficiency.Meanwhile,gastrointestinal heat accumulation syndrome affects Cx43 protein expression and its mRNA expression,and further affects the contraction of gastrointestinal smooth muscle,leading to gastrointestinal dyspnea. |
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