Experimental Study Of Magnetic Induction Hyperthermia Combined With Drugs On Small Cell Lung Cancer H446 Cells

Objective:This subject discusses the effect of magnetic induction hyperthermia on small cell lung cancer H446 cells and verifies whether magnetic induction hyperthermia has inhibitory effect on the proliferation of H446 cells.Magnetic induction hyperthermia will be combined with Xiaoaiping and Irinotecan(CPT-11)to act on H446 cells respectively to clarify the effects of magnetic induction hyperthermia and different drugs on cell proliferation,apoptosis and cell cycle.The purpose of this study is to explore the mechanism of magnetic induction hyperthermia combined with drug intervention H446,and to provide reference for clinical treatment of small cell lung cancer.Methods:1.Parameter determination of magnetic microspheresUse temperature measurement software to record the temperature increasing performance of the magnetic microsphere culture medium with different mass concentrations at 300 kHz and 40 Gs alternating magnetic field;the prepared magnetic microsphere extracts with different mass concentrations are used to continuously culture H446 cells 3 Days,detect the cell viability OD value.2.Magnetic induction hyperthermia combined with Xiaoaiping on H446 cellsThe effect parameters of H446 cell intervention by magnetic induction hyperthermia were determined to be 300 kHz,40 Gs,and 30 min.This experiment was set up as normal control group,magnetic induction group,Xiaoaiping group,and magnetic induction combined Xiaoaiping group.The CCK-8 method was used to determine the OD value of each group,and the cell suppression rate of each group was calculated;the flow cytometry was used to detect the cells of each group Cycle and apoptosis.3.Magnetic induction hyperthermia combined with CPT-11 acts on H446 cellsThe effect parameters of H446 cell intervention by magnetic induction hyperthermia were determined to be 300 kHz,40 Gs,and 30 min.This experiment was set up as a control group,magnetic induction group,CPT-11 group,combined combination of magnetic induction and CPT-11,using the CCK-8 method to determine the OD value of each group,calculating the cell inhibition rate of each group;using flow cytometry to detect the cells of each group Apoptosis and cell cycle conditions.Results:1.The temperature rise of magnetic microspheres can reach 45℃ within 30min at 300kHz,40Gs magnetic field and 60mg/mL mass concentration.H446 cells were continuously cultured with magnetic microsphere extract,and the cell viability was all higher than 90%,showing good biocompatibility.2.Magnetic induction hyperthermia combined with anticancer drugs acts on H446 cells:in the magnetic induction combined with anticancer drugs group,the number of H446 cells is obviously reduced,the gap becomes larger,the number of suspended cells increases,and the cell body shrinks obviously;The 24-hour inhibition rate of H446 cells treated by magnetic induction combined with xiaoaiping group was(47.72 ± 0.52)%(p<0.01),and the apoptosis rate was(40.06 ±1.00)%(p<0.01).Cell cycle results:in the magnetic induction group and xiaoaiping group,G0/G1 phase cells decreased,G2/M phase and s phase cells increased,while in the magnetic induction combined xiaoaiping group,the percentage of G0/G1 phase cells decreased compared with the control group,and the percentage of G2/M phase cells was(20.49 ± 3.56)%(p<0.01),with no significant difference in s phase changes.3.Magnetic induction hyperthermia combined with CPT-11 acts on H446 cells:the magnetic induction combined with CPT-11 group showed more obvious decrease in the number of adherent cells,increased suspension cells,weakened adherence performance of adherent cells,contraction of cell soma,and increased cell spacing.The cytostatic rate of the combined group was significantly increased,being(36.58 ± 2.1)%(P<0.01).Apoptosis results:The total apoptotic rate of the combined group was(28.9 ± 3.64)%,(P<0.01)significantly higher than that of the control group,magnetic induction group,and CPT-11 group.Cell cycle results:The proportion of cells in the G2/M phase of the combined group was significantly higher than that of the control group,but lower than that of the CPT-11 group and the magnetic induction group;the proportion of cells in the S phase was higher than the control group and lower than that in the magnetic induction group;and the proportion of cells in the G0/G1 phase was low.Yu Yizhao group.The percentage of G2/M cells in the CPT-11 group was significantly higher than that in the control group.Conclusion:1.The magnetic microsphere extract has little effect on the activity of H446 cells,has good heating capacity and temperature maintenance capacity,and has the potential to be used as a magnetic induction hyperthermia medium.2.Magnetic induction hyperthermia combined with Xiaoaiping intervened in H446 cells showed an additive effect,significantly increasing the inhibition of H446 cell proliferation and inducing apoptosis of H446 cells;in terms of cell cycle,magnetic induction hyperthermia group,Xiaoaiping group and combination group can all treat The cells were blocked in the G2/M phase,but the combined application showed no obvious synergistic cycle blocking effect.3.The combination of magnetic induction hyperthermia and CPT-11 showed an additive effect,which could inhibit the proliferation of small cell lung cancer H446 cells and promote their apoptosis;the combination of magnetic induction and CPT-11 did not show an obvious synergistic effect on cell cycle arrest.4.The experimental study of magnetic induction hyperthermia combined with drug intervention on H446 cells provides a new combination therapy scheme for the treatment of small cell lung cancer,and also provides the possibility for magnetic induction hyperthermia to further explore the treatment of deep tumors.