Study On The Effect Of Magnetic Induction Hyperthermia Combined With Drugs On MCF-7 Cells

Purpose:Based on previous research,this experiment will further study the effect of magnetic induction hyperthermia(MIH)combined with epirubicin(EPI)and Kanglaite injection on human breast cancer MCF-7 cells.Through the detection of the heating performance and biocompatibility of human breast cancer MCF-7 cells with different concentrations of magnetic microspheres,the optimal magnetic medium concentration was screened to evaluate the feasibility and safety of magnetic induction thermotherapy magnetic medium.Magnetic induction hyperthermia combined with epirubicin and Kanglaite injection to interfere with MCF-7 cells,and clarify the effects of combined effects on MCF-7 cell viability,cell cycle distribution,and apoptosis rate.The effects of magnetic induction hyperthermia on the proliferation of MCF-7 cells and the feasibility of breast cancer treatment were explored.Method:1.Measurement of magnetic microspheres in vitro heating performanceEach group of magnetic microspheres with different mass concentrations was placed in a 300kHz 40Gs alternating magnetic field device.The temperature recording system and temperature display were used to measure and record the relationship between the temperature rise performance and time of the magnetic microspheres in vitro,and draw the heating curve.2.Detection of biocompatibility between magnetic microspheres and MCF-7 cellsMCF-7 cells in logarithmic growth phase were cultured with the magnetic microsphere extracts of each group for 24 hours,48 hours,and 72 hours,and the cell proliferation activity was detected by the MTT method at corresponding times to observe the cell morphological changes.3.Effect of magnetic induction hyperthermia combined with epirubicin on MCF-7 cellsMTT method was used to detect the inhibition rate of epirubicin on MCF-7 cells by different concentrations,and the working concentration of epirubicin was selected.Experimental settings:control group,magnetic induction hyperthermia group,epirubicin group and combined group,each group took corresponding intervention measures,magnetic induction group and combined group were placed in 300kHz 40Gs alternating magnetic field equipment for 30min and returned to the incubator.The culture was continued for 24 hours,and the cell inhibition rate,apoptotic rate,and cell cycle of each group were measured by MTT method and flow cytometry.The effects of magnetic induction thermotherapy combined with epirubicin on MCF-7 cells were compared and analyzed.4.Effect of magnetic induction hyperthermia combined with Kanglaite injection on MCF-7 cellsMTT method was used to detect the inhibition rate of Kanglaite injection at different concentrations on MCF-7 cells,and the working concentration of Kanglaite injection was selected.Experimental settings:control group,magnetic induction hyperthermia group,Kanglaite group and combined group,each group took corresponding intervention measures,magnetic induction group and combined group were placed in 300kHz 40Gs alternating magnetic field equipment for 30 minutes,and then returned to the incubator.The culture was continued for 24 hours,and the cell inhibition rate,apoptotic rate,and cell cycle of each group were measured by MTT method and flow cytometry.The effects of magnetic induction thermotherapy combined with Kanglaite injection on MCF-7 cells were compared and analyzed.Result:1.The magnetic microspheres selected in this experiment have acceptable heating performance,are toxic to MCF-7 cells?1 grade,and have good biocompatibility.Because the high temperature of 42?-45? can kill tumor cells and there is almost no damage to normal cells.according to the temperature rise curve,this experiment uses magnetic microspheres with a concentration of 65mg/ml as the magnetic medium for alternating magnetic field induction heating.2.In a 300kHz 40Gs alternating magnetic field device,after the 65mg/ml magnetic microspheres were applied to MCF-7 cells for 30 minutes,the relative proliferation rate of the cells decreased to(84.18±2.41)%,(P<0.01);magnetic induction hyperthermia Promote cell apoptosis,the proportion of early withering and late withering cells is(19.00±4.62)%,(P<0.05),and MCF-7 cells can be blocked at G2/M phase.3.In this experiment,the working concentration of epirubicin was 13.14 ? mol/l.After MCF-7 cells were combined with magnetic induction hyperthermia and epirubicin,the cell proliferation rate decreased to(38.51±1.26)%,(P<0.05);The combination group can promote the apoptosis of MCF-7 cells,and the early and late withering cells are significantly increased.The ratio of early and late withering cells is(61.47±2.12)%,(P<0.01);7The cell cycle has a blocking effect,which can block cells in GO/G1,G2/M phases.4.In this experiment,the working concentration of Kanglaite injection was 400 ?g/ml.After the combination of magnetic induction hyperthermia and Kanglaite injection on MCF-7 cells,the cell proliferation rate decreased to(55.88±2.87)%,(P<0.01);The combination group could promote the apoptosis of MCF-7 cells.The proportion of early withering and late withering cells was(40.60±3.88)%,(P<0.01).The combination group had less effect on MCF-7 cell cycle.Conclusion:The magnetic microspheres and magnetic media used in this experiment proved to be safe and feasible for MCF-7 cells in vitro cell experiments.Magnetic induction hyperthermia can inhibit the proliferation of MCF-7 cells,promote cell apoptosis,and slightly arrest the MCF-7 cell cycle in the G2/M phase;magnetic induction hyperthermia has a significant synergistic anti-MCF-7 cells effect on epirubicin against the proliferation of MCF-7 cells,can significantly promote the apoptotic effect of epirubicin on it,and can increase the arrest cycle of MCF-7 cells,blocking the cells in the GO/G1 and G2/M phases.Magnetic induction hyperthermia can increase Kanglaite injection's inhibitory effect on MCF-7 cell proliferation and promote cell apoptosis,but it has no significant effect on the cell cycle arrest of Kanglaite injection.Many in vitro experiments have proved the effectiveness and safety of the new magnetic induction hyperthermia technology for breast cancer cell therapy,which has opened up new ideas for clinical treatment of breast cancer.