The Changes Of Liver Tissue Pathology And Energy Metabolism In A Model Of Chronic Atrophic Gastritis Combined With Disease And Syndrome

1 ObjectiveIn the presented subject,the hepatic pathological and functional changes were observed on chronic atrophic gastritis(CAG)rat model with spleen deficiency sydrome.In further,the expression of proteins related to PI3K/Akt signaling pathway were examined to figure out the preliminary mechanisms on reduced energy metabolism of CAG rat model with spleen deficiency sydrome.2 MethodsThe experiment is mainly consisting of four parts.(1)Male Wistar rats were used as experimental materials,Four comprehensive factors including N-methyl-N-nitro-nitrosoguanidine(MNNG),sodium salicylate,ranitidine and starvation were used to make model of CAG.To observe phenotype of rats at the 28th and 40th weeks after modeling and determine the syndrome.(2)The level of AST,ALT,GLU,CHO,TG,HDL,LDL in serum was measured by automated biochemical instrument.These concentrations changes in peripheral blood to study the relationship between hepatic function and glucometabolic and lipid metabolism.(3)Hepatic pathological changes in rats with CAG and TCM syndrome were observed by H&E staining;periodic acid-Schiff(PAS)reaction and transmission electron microscope(TEM).(4)The expression of PI3Kp85alpha,Akt,COX IV of rats at the normal group,the 40thweek of modeling group in rat liver were performed by immunohistochemical method.The expression of PI3Kp85α,Akt of rats at the normal group,the 40th week of modeling group in rat liver were detected by Western blot.To observe the effect of indicator changes on the material and energy metabolism of CAG.3 ResultsThe rats showed spleen deficiency syndrome at 28th weeks after modeling,while the rats showed spleen asthenia and blood stasis at 40th week after modeling.Compared with the control group,the level of ALT and AST significantly increased at 28th week after modeling.The level of ALT still significantly increased at 28th week after modeling,while the level of AST was not significantly changed at 40th week after modeling.Compared with the control group,the level of GLU significantly increased at 28th and 40th week after modeling.Compared with the control group,the level of TG,CHO,HDL significantly decreased at 28th and 40th week after modeling,while the level of LDL was not significantly changed at 28th week after modeling.Compared with the 28th week group,the level of LDL significantly increased,while the level of TG,CHO,HDL decreased,but there was no significant difference.Compared with the control group,pathological changes indicated that hepatic cords were disorder and hepatocytes were swollen at 28th week after modeling while the hepatocytes were shrank,hepatic sinusoid became smaller and the amount of inflammatory cells increased at 40th weeks after modeling by HE staining;It is showed that synthesis and storage of glycogen both decreased at 28th and 40th weeks after modeling by PAS reaction;It is also showed that mitochondria in hepatocytes were swollen and denatured at 28th and 40th weeks after modeling by TEM.Compared with the normal group,the rats in the 40th weeks after modeling higher PI3Kp85a protein content,lower Akt.Compared with the normal group,the rats in the 40th weeks after modeling had the phenomenon of low expression of COX Ⅳ.4 ConclusionThe four-factor comprehensive modeling method can be used to establish the animal model of CAG.There were significant changes in liver function,glycometabolism,lipid metabolism and histopathology.content as follow:the level of transaminase increased,hepatic glycogen synthesis and reserve decreased,blood glucose,cholesterol,triglyceride and high density lipoprotein increased,low density lipoprotein decreased,mitochondria were swollen,number decreased and oxidative phosphorylation function was weakened.CAG and TCM syndrome is related to low expression of PI3K/Akt signaling pathway and COX Ⅳprotein content.