Dynamics Of Mitochondrial Nucleic Acid By Super-resolution And Lifetime Imaging Of Fluorescent Probes

In mammalian cells,the transmission of genetic information basically follows the central dogma,usually starting with DNA,then flowing to RNA,and finally to protein.Cell state,development and differentiation are all regulated by DNA.Mitochondria are the only organelles outside the nucleus that carry genetic information.Mitochondria are semi-autonomous organelles that can independently perform DNA replication,DNA transcription,RNA translation,and protein synthesis.Mitochondria are also the main place for aerobic respiration in cells,providing energy for cells.Submitochondrial cells participate in a number of growth and differentiation activities,participate in cell differentiation,cell apoptosis,cell information transmission,and have the ability to regulate cell growth and regulate cell cycle.Mitochondria have a negative transmembrane potential(??m),which is one of the important functions of mitochondria,which leads to the efficient accumulation of cationic dyes in mitochondria driven by ??m.In principle,the direct binding of mitochondrial fluorescent probes to mitochondrial DNA in situ can minimize the interference of nu DNA and generate a fluorescent response.In this paper,we report a probe for locating mitochondria.After stably binding to mitochondrial nucleic acid,super-resolution imaging and fluorescence lifetime imaging were used to observe the distribution of different nucleic acids in the mitochondria and the lifespan changes when the mitochondria changed their morphology.1.We synthesized a positively charged small molecule that can target mitochondria.Free small molecules can dissipate energy through the single bond rotation and the isomerization of the azo bond.When combined with the nucleic acid,the single bond rotation and the isomerization of the azo bond in the molecule are inhibited,and the molecule is released from the excited state When the form of the photon returns to the ground state,the molecule emits intense fluorescence.2.Using super-resolution microscopy to co-localize with Mito tracker deep red dye,it is found that the mitochondrial deep red dye binds to mitochondrial membrane proteins and is located on the mitochondrial membrane,while our fluorescent probe is located in the mitochondrial matrix,and mitochondria Nucleic acid binding.