| At present,the medical level and diagnostic technology have been rapidly developed,but the morbidity and mortality of diseases caused by viruses are still at a high level,which is one of the important reasons threatening human health and life safety.Prompt detection and accurate detection of related diseases in the early stage are crucial to improve survival.Zika virus(ZIKV)is a single-stranded RNA virus belonging to the family Flaviviridae,with high morbidity and mortality.So far,no effective drugs or vaccines have been developed to specifically treat this type of disease,and more sensitive assays for this type of pathogenic virus need to be developed.Based on this,a new biosensor based on inductively coupled plasma mass spectrometry(ICP-MS)combined with nanoparticle labeling was constructed for the detection of biomacromolecules,and its application in the detection of Zika virus nucleic acid fragment(ZIKV-RNA)was successfully realized.This paper is mainly divided into four chapters:The first chapter is the introduction,which mainly summarizes the significance and detection methods of ZIKV,the advantages of ICP-MS,and the scientific problems and main research contents solved in this paper.In the second chapter,ICP-MS was used to detect heavy metals in vegetables and fruits and to evaluate their safety.Vegetables and fruits are an important part of human diet.They contain nutrients closely related to human health.However,existing studies have shown that vegetables and fruits are often polluted by heavy metals.A total of 78 representative samples of 26 stem vegetables,17 leaf vegetables,27 fruit vegetables and 8 fruits were collected from retail stores and local markets in Shandong Province.The contents of lead(Pb),arsenic(As),cadmium(Cd)and mercury(Hg)in fruit and vegetable samples were determined by ICP-MS.The pollution of four heavy metals in vegetables in Shandong Province was analyzed based on the over-standard rate and pollution index method.Exposure assessment and risk coefficient model were used to evaluate the safety of heavy metals in vegetables.The results showed that the order of the average heavy metal contents in the vegetable samples of Shandong Province was As follows:Pb>As>CD>Hg,and the order of the average heavy metal contents in the fruit samples of Shandong Province was As follows:Pb>Hg>As=Cd.The content of heavy metals in the samples was generally at a low level.The over-standard rate of Hg in the vegetable samples was 1.43%,and the over-standard rate of the other samples was 0.The comprehensive pollution index of oilseed vegetables reached the pollution warning line,and the other vegetable samples were at the safe level.The heavy metal Pb exposure in vegetables of Shandong residents was more obvious.The risk coefficient of heavy metals ingested by Shandong residents through eating vegetables is all less than 1,which is within the safe range and will not pose a threat to the health of residents.In the third chapter,a new biosensor based on ICP-MS combined with nanoparticle labeling for biomacromolecule detection was constructed,and its application in the detection of ZIKV-RNA was successfully realized by using ICP-MS and fluorescence dual-mode detection.In this method,the MB-ssDNA-[signal DNA-CdSe QDs-link DNA]n complex was formed by hybrid chain reaction(HCR)amplification,and the target sequence was added to generate the MB-ssDNA-target and the signal was released to realize sensitive detection.ICP-MS and fluorescent dual-mode signal detection were used to realize mutual verification of the analysis results and improve the accuracy of the analysis results.The results show that the biosensor has good application value,which expands the application of biosensor technology in ZIKV detection,and also provides a new idea for high specificity and sensitivity detection of ZIKV.The fourth chapter is the conclusion and prospect,which summarizes and prospects the research results of this paper. |
PDF Full Text Request