Study On Microbial Diversity And Establishment Of Bacterial Resource Bank In Wine Region Of Helan Mountain In Ningxia

Taking ningxia helan mountain XXQ?YNX?QTX?SZS of vineyard soil collected four areas,grapes,the research object,through the high flux illumina Mi Seq sequencing analysis of microbial diversity in samples,screening of liquor-making microbes and excellent characteristics of wine yeast,to explore the factors influencing the ability of yeast to produce acid,with the screen strains wine and quality analysis,finally realizes the ningxia helan mountain foothill characteristics of improving the quality of the wine.The main research results are as follows:(1)The results of high-throughput sequencing showed that,based on the similarity of 97%,fungi obtained 4 phyla and 1107 OTU at the phylum level,while bacteria obtained 11 phyla and 4630 OTU at the phylum level.At the genus level,the dominant fungi in grape were Aspergillus,Alternaria,Exobasidum,Aureobasidium and Cladosporium,while the dominant fungi in soil were Gibberella,Gliomastix,Coprinellus,Fusarium and Chaetomium.The dominant bacteria in grape were Chryseobacterium,Mesorhizobium,Pseudomonas,Microbacterium and Acinetobacter,while the dominant bacteria in soil were Arthrobacter,Pontibacter,Massilia,Skermanella and Candiatus-Nitrocosmicus.The study on microbial diversity in vineyards at the eastern foot of Helan Mountain provides a theoretical basis for the utilization and development of characteristic microorganisms in vineyards.(2)on the basis of microbial diversity information to build a about ningxia helan mountain microbial diversity of microbial library,library is mainly to establish the bacteria by microorganism separation was achieved by methods such as culture medium,and the physiological and biochemical test,the apparent morphology analysis and molecular biology method implementation microbial identification,make preliminary shaping microbial library.Microorganisms can be directly divided into different types through the preliminary research,and then the application research of microbial library can be carried out.At present,the bacterial bank contains 146 strains of yeast,93 strains of bacteria and 36strains of molds,basically covering the relatively common microorganisms in the vineyard.And we understand the various microorganisms through the experiment of the morphological characteristics,growth characteristics and implemented for the classification of microorganisms,the identification of the bacteria in the library part focus on microorganisms,these microbes included brewing yeast Saccharomyces cerevisiae,dell has a spore mother Torulaspor,delbrueckii,grape juice has the spore mother Hanseniaspora uvarum sweet and sour coli,bacillus,Portugal,penicillium,aspergillus and so on,they are common in typical microorganism for wine production.(3)46 strains of Saccharomyces cerevisiae and 16 strains of bacteria were isolated by 26S r DNA and 16S r DNA Through fermentation performance,the transforming ability of malic acid and tolerance test,select the performance of good wine brewing yeast(Saccharomyces cerevisiae)10 strains of Bacteria by natural fermentation wine sample source,hidden at the center of the preservation of commercial species for comparison,6057 down to 16%alcohol,100 mg/L of SO₂ concentration,p H value of 3.0 under the condition of normal growth,and growth condition of lactic acid Bacteria,malic acid,lactic acid Bacteria(Malolactic Bacteria)4 strains,Among them,L2,L3 and L16 are Oenococcus L4are Lactobacillus plantarum.It was found that L2 and L3 could be used as excellent malolactic acid fermentation strains for further study after brewing experiments on these four strains.