Effects Of Triple Eradication Therapy On Intestinal Flora In Asymptomatic People With Helicobacter Pylori Infection

Objective: To evaluate the effects of triple eradication therapy on the diversity and community composition of intestinal flora in asymptomatic people with Helicobacter pylori infection(H.pylori).Methods: The high-throughput Illumina sequencing technology was used to examine faecal samples from 29 H.pylori infected subjects,before the H.pylori eradication treatment(Omeprazole,Amoxicillin and Clarithromycin)and 10 days after,respectively.16 S r RNA V3 variable regions were sequenced and analyzed to investigate the effects of H.pylori eradication treatment on the diversity and composition of intestinal flora in 29 asymptomatic subjects with Helicobacter pylori infection.Result: Fifty-eight samples from 29 subjects have a total of 363 OTUs obtained by high-throughput sequencing method,of which 277 OTUs are common.There are 80 particular OTUs in the samples before treatment(group b),while it is reduced to 6particular OTUs after treatment(group a).In the diversity of flora,the shannon index of intestinal flora after treatment(group a)was found to be significantly lower than that before(group b)(z=-3.266,p = 0.001).In terms of the structure and composition of intestinal flora,on the phylum level,the relative abundance of Proteobacteria in group a is significantly higher than that in group b(p=0.0129),while the relative abundance of Actinobacteria in group a is lower than that in group b(p=0.0029).On the genus level,the relative abundance of Bifidobacterium**,Lachnospira **,Ruminococcus* and Sutterella** are reduced and that of Escherichia/Shigella ** is increased after treatment(group a)(*p<0.05,**p<0.01).Conclusions : H.pylori eradication treatment also can change the diversity and community structure and composition of intestinal flora when eradicating H.pylori,accompanied by a reduction of intestinal probiotics and an increase of the opportunistic pathogens.This change in the intestinal flora may cause the disturbances of intestinal flora and may even lead to the development of diseases.Our study may provide some evidence and information for Chinese gastroenterologists to help them better manage the asymptomatic H.pylori infected people.