The Difference Of The Whole Transcriptome Expression Profile Between Acute Pancreatitis And Recurrent Acute Pancreatitis Was Studied By High Throughput Transcriptome Sequencing Technology

Objective: To explore the potential molecular pathogenesis of miRNA and lncRNA related acute pancreatitis(AP)and recurrent acute pancreatitis(RAP),The interaction network between miRNA and lncRNA was constructed to contribute to the exploration of therapeutic methods for pancreatic diseases at the gene level and the research and development of new drugs.Methods: In this project,miRNAs and lncRNAs in blood samples from control group(5 cases),acute pancreatitis(AP)patients(5 cases)and recurrent acute pancreatitis(ARP)patients(5 cases)were compared and studied by high-throughput transcriptome sequencing(RNA-seq)technology.Results: Compared with the control group,a total of 2780 different mRNAs were obtained in AP group,including 1807 up-regulated genes and 973down-regulated genes.There were 1065 differentially expressed lncRNAs,including 592 up-regulated and 473 down-regulated lncRNAs.There were 37 differentially expressed miRNAs,17 of which were up-regulated and 20 of which were down-regulated.A total of 1195 differentially expressed mRNAs were obtained in the ARP group,including 919 up-regulated genes and 276down-regulated genes.409 differentially expressed lncRNAs were found,of which 307 lncRNAs were up-regulated and 102 were down-regulated.There were 13 miRNAs with differences,including 11 miRNAs with up-regulated expression and 2 miRNAs with down-regulated expression.There were 909 differentially expressed mRNAs,315 differentially expressed lncRNAs and 12 differentially expressed miRNAs in the two groups,and the expression trend of each differentially expressed gene in the two groups was consistent.Differential genes co-expressed by lncRNA-mRNA are involved in immune-related biological processes such as neutrophil degranulation and adaptive immune response,and are also enriched in such pathways as Metabolic pathways and cytokine-cytokine receptor interaction.By differential expression analysis of ARP-specific genes,286 mRNAs,94 lncRNAs and 1 miRNA(hsa-miR-342-5p)were found to be down-regulated.LncRNA-mRNA co-expression analysis revealed that the biological processes of the differentially expressed genes were involved in the methionine metabolic process and concentrated in the following pathways: Oxidative phosphorylation,metabolic pathways,etc.CONCLUSIONS: LncRNA LUCAT1/ NEAT1-miR-16-2-3-P-AATK/ENTPD1/CREB5 may be involved in the pathogenesis of pancreatitis in the common ceRNA network.However,cRNA network analysis for miR-342-5p suggested that lncRNA DGCR9/ ASAP1-IT2-miR-metabolic pathway342-5p-ABCC5/MAP2K6 K network may be involved in the pathogenesis of ARP disease.Conclusion: Among the common ceRNA networks,lncRNA LUCAT1/NEAT1-miR-16-2-3p-AATK/ENTPD1/CREB5 regulatory networks may be involved in the pathogenesis of pancreatitis.The cRNA network analysis for miR-342-5p suggested that lncRNA DGCR9/ASAP1-IT2-miR-342-5p-ABCC5/MAP2K6 K network might be involved in the pathogenesis of ARP disease.