| Objective:Asprosin,a novel adipokine,is closely related to diabetes and obesity.The correlation between serum asprosin level and visceral fat area,glucose and lipid metabolism in newly diagnosed type 2 diabetes patients was investigated in this study,so as to provide more clinical basis for the diagnosis and treatment of type 2 diabetes and abdominal obesity.Methods:According to the diabetes diagnostic criteria proposed by the WHO Diabetes Committee in 1999,a total of 110 patients with type 2diabetes,including 66 males and 44 females,were enrolled in this study from June 2020 to January 2021 in the Department of Endocrinology of the Second Affiliated Hospital of the University of South China.In addition,the selection of the same age and sex ratio of matched 68 healthy people were selected as normal control group,including 34 males and 34 females,according to the abdominal obesity diagnosis standard putted forward by the Japanese association of obesity in 2002,each group was divided into abdominal obesity and non abdominal obesity group,namely of type 2 diabetes abdominal obesity group(T2DM-ob),type 2 diabetes non abdominal obesity group(T2DM-nob),normal glucose tolerance abdominal obesity group(NGT-ob),normal glucose tolerance non abdominal obesity group(NGT-nob).All subjects were asked in detail about basic information,past history,medication history,personal history,family history,etc.All subjects underwent routine physical examinations,including blood pressure(BP),height(HT),weight(WT),waist circumference(WC),and hip circumference(HC).To collect all the object of study on an empty stomach blood samples used for detecting fasting plasma glucose(FPG),fasting insulin(FIns),glycosylated hemoglobin(Hb Alc),triglycerides(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),alanine aminotransferase(ALT),aspertate aminotransferase(AST),creatinine(Cr),urea nitrogen(BUN),uric acid(UA),C-reactive protein(CRP),glutamic acid decarboxylase antibody(GADA).Serum levels of asprosin and leptin in all subjects were determined by enzyme-linked immunosorbent assay(ELISA).Blood samples were collected from all subjects 2 hours after glucose load for the detection of 2-hour blood glucose(2h-PG)and 2-hour insulin(2h-Ins).Body mass index(BMI),waist-hip ratio(WHR)and homeostasis model were calculated to evaluate insulin resistance index(HOMA-IR).The visceral fat area(VFA)of all subjects was measured by dual-energy X-ray absorption method.Results:1.Comparison of clinical data and metabolic indexes among the four groups:compared with T2DM-nob group: BMI,WC,HC,VFA,TG,TC,LDL-C and asprosin were significantly increased,while HDL-C was significantly decreased in T2DM-ob group(P<0.05);compared with NGT-nob group: BMI,WC,HC,VFA,TG,TC,LDL-C and asprosin were significantly increased,while HDL-C was significantly decreased in NGT-ob group(P<0.05);compared with NGT-ob group : FPG,FIns,HOMA-IR,Hb Alc,asprosin,leptin were significantly increased in T2DM-ob group(P<0.05);compared with NGT-nob group: FPG,FIns,HOMA-IR,Hb Alc,asprosin,leptin were significantly increased in T2DM-nob group(P<0.05).2.Three groups were divided according to serum asprosin level: T1group(asprosin concentration<7.35ng/ml),T2 group(asprosin concentration between 7.35 and 9.50ng/ml),T3 group(asprosin concentration>9.50ng/ml),and the comparison of clinical data and metabolic indexes among the three groups: compared with T1 group,VFA,FPG,FIns,HOMA-IR,Hb Alc,leptin,T2DM(%)were significantly increased in T2 and T3 groups(P<0.05),BMI,TG,TC,LDL-Cand abdominal obesity(%)in T3 group were significantly increased;Compared with T2 group,T2DM(%)in T3 group were significantly increased(P<0.05).3.Correlation analysis of asprosin,leptin and VFA with clinical data and metabolic indexes: Pearson correlation analysis showed asprosin was significantly positively correlated with BMI,WC,HC,FPG,HOMA-IR,Hb Alc,TG,TC,LDL-C,VFA and leptin,and negatively correlated with HDL-C(P<0.05);there was a significant positive correlation between leptin and FPG,FIns,HOMA-IR,Hb Alc,TG,LDL-C,Cr,CRP,VFA,asprosin(P<0.05);VFA was positively correlated with systolic blood pressure,diastolic blood pressure,BMI,WC,HC,TG,TC,LDL-C,UA,asprosin and leptin,and negatively correlated with HDL-C(P<0.05).4.Using VFA as the dependent variable,Logistic multiple regression analysis showed that BMI,TG,LDL-C and asprosin were independent risk factors for VFA,while HDL-C was a protective factor for VFA(P<0.05).5.Diagnostic value of serum asprosin in abdominal obesity: The best cutoff value of asprosin in the diagnosis of abdominal obesity was10.495ng/ml,and the area under the curve was 0.676(P<0.001),the sensitivity was 34.1%,and the specificity was 91.1%.Conclusions:1.Serum asprosin level was significantly increased in newly diagnosed T2DM patients and patients with abdominal obesity.2.Asprosin is closely related to the VFA,glucose and lipid metabolism.And asprosin is an independent risk factor for VFA.As a new adipokine,asprosin may be involved in the occurrence and development of T2DM and abdominal obesity. |
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