Calcium Dobesilate Inhibits Proliferation And Migration Of RF/6A Cells Induced By High Glucose And Related Mechanisms

Objective: To observe the inhibitory effect of calcium dobesilate on the proliferation and migration of monkey choroid-retinal endothelial cells(RF/6A)induced by high glucose,and to preliminarily explore its possible mechanism.Methods: The RF/6A cells were cultured and divided into low glucose group(NG group),high glucose group(HG group),high glucose plus different concentrations of calcium dobesilate group(HG+50μmol/L group,HG+100μmol/L Group,HG+200μmol/L group).The CCK8 method was used to detect the proliferation activity of each group of cells;the Transwell experiment was used to detect the migration of each group of cells;the Western-blot was used to detect the expression of LATS1,YAP1,and TEAD1 protein in each group of RF/6A cells.Results: Compared with the NG group,the proliferation activity of RF/6A cells in the HG group increased(P<0.01).Different concentrations of calcium dobesilate inhibited the proliferation of RF/6A cells induced by high glucose(P < 0.01),the inhibition rate of proliferation activity of HG+50μmol/L group,HG+100μmol/L group,and HG+200μmol/L group were(9.3±0.9%),(22.8±1.3%),(31.0±1.5%),respectively;the difference between different doses of calcium dobesilate groups was statistically significant(P<0.05).Compared with the NG group,the number of migrating cells in the HG group increased(P<0.01).Different concentrations of calcium dobesilate inhibited the migration of RF/6A cells induced by high glucose(P<0.01),The cell migration inhibition rates of the HG+50μmolgroup,HG+100 μ mol/L group,and HG+200 μ mol/L group were(33.2±0.8%),(44.3±0.9%),(58.9±1.2%),respectively;the difference between the different doses of calcium dobesilate groups was statistically significant(P<0.05).The LATS1 protein expression levels in the NG group,HG group,HG+50μmol/L group,HG+100μmol/L group,and HG+200μmol/L group were(1.14±0.047),(0.64±0.027),(0.73±0.023),(0.82±0.041),(0.91±0.042);YAP1 protein expression in NG group,HG group,HG+50μmol/L group,HG+100μmol/L group,HG+200μmol/L group were(0.49±0.030),(0.58±0.050),(0.49±0.036),(0.40±0.029),(0.29±0.037);NG group,HG group,HG+50μmol/L group,HG+100μmol/L group,HG+200μmol/L group TEAD1 protein expression levels were(0.53±0.034),(0.61±0.050),(0.50±0.029),(0.30±0.018),(0.21±0.032).Compared with the NG group,the YAP1 and TEAD1 protein expression in the HG group increased(P<0.05),and the LATS1 protein expression decreased(P<0.01);the YAP1 protein expression in the calcium dobesilate group at different concentrations was lower than that in the HG group(P<0.05),TEAD1 protein expression was lower than HG group(P<0.01),LATS1 protein expression was higher than HG group(P<0.05);the difference between different doses of calcium dobesilate groups was statistically significant(P<0.05).Conclusion: Calcium dobesilate inhibits the proliferation and migration of RF/6A cells induced by high glucose,and its mechanism may be related to the regulation of Hippo signaling pathway.