Effects Of Dexmedetomidine On Intestinal Injury Caused By CO₂ Pneumoperitoneum In Gynecological Laparoscopic Surgery

Objective:To discuss the intestinal injury about CO₂ pneumoperitoneum on gynecological laparoscopy and the effects of DEX.Methods:We selected 40 of our patients with multiple uterine fibroids.All patients planed to have LM,they were divided into group C（Saline solution groups）and D（DEX groups）randomly,20 cases in each group,Patients in Group D were given Dex 0.5ug/kg within 10 minutes before induction.After intubation,we need to change the dosage of DEX to 0.2 ug/kg/h and to maintain until the end of pneumoperitoneum.Patients in group C were given equivalent quantity of saline solution in the same way.We need to choose three time points:T1（10 minutes before induction）,T2（one hour after pneumoperitoneum）and T3（24 hours after pneumoperitoneum）and we need to draw venous blood from two groups of patients.We need to determine the levels of Claudin-1 and Dao in serum.And finally,we should observe and compare the changes of Claudin-1 and Dao between group C and D in order to test the influence of DEX.Results:Claudin-1 was lower in T2 and T3 than in T1（p<0.01,p<0.05）,and DAO was higher in T2 and T3 than in T 1（p<0.01,p<0.05）in group C.Claudin-1 and DAO in group D had no significant difference at different time points,and Claudin-1 in T2and T3 was higher than that in group C（p<0.05）,and Dao was lower in T2 and T3than that in group C（p<0.01,p<0.05）.Conclusion:CO₂ pneumoperitoneum in gynecological laparoscopy may cause intestinal injury.The expression of claudin-1 protein was up-regulated after preconditioning.The content of DAO released into the blood was decreased,the results showed that Dex intervention could prevent and cure intestinal injury caused by CO₂ pneumoperitoneum.