Association Between MiR-30a/Snai1 Gene Polymorphisms And The Risk Of Diabetic Kidney Disease

Background:Diabetic kidney disease is a leading cause of end-stage kidney disease worldwide,its incidence is still increasing and the precise mechanism is not yet fully understood.This study aimed to investigate the possible association of miR-30 a and its potential target gene,SNAI1,polymorphisms with diabetic kidney disease in patients with type 2diabetes mellitus.Methods:This case-control is study included 240 type 2 diabetes mellitus patients with diabetic kidney disease and 280 patients without diabetic kidney disease.Genotyping of miR-30 a and SNAI1 polymorphisms were performed by allelic discrimination assay with Taq-Man-MGB probes.Results:The results demonstrated that the CC genotype of rs2222722 in miR-30 a was associated with an increased risk of diabetic kidney disease in Chinese type 2 diabetes mellitus patients(OR = 2.81,95% CI 1.58-4.97,p < 0.001).Stratified analyses revealed that the miR-30 a CC genotype was strongly associated with an increased risk of diabetic kidney disease in subjects who were young(< 65 years),male,and had a low body mass index,as well as those with poor glycemic control.What’s more,CC genotype carriers were more likely to exhibit decreased estimated glomerular filtration rate levels and urinary protein production.In addition,the GG genotype of SNAI1 was also associated with the development of diabetic kidney disease,and the risk was 1.73 times higher than that in AA+AG genotype carriers(95% CI 1.01-2.96,p = 0.046).Multiple logistic regression analysis showed that the miR-30 a CC and SNAI1 GG genotypes were indispensable and dangerous contributing factors to the development of diabetic kidney disease.Conclusions: The CC genotype of miR-30 a rs2222722 and GG genotype of SNAI1 rs1543442 might be associated with diabetic kidney disease risk in Chinese type 2 diabetes mellitus patients.And miR-30 a rs2222722 CC genotype and reduced glomerular filtration rate and urine protein production relationship is also cannot be ignored.