| Objective Large area burn patients usually occurs sequelae of hypertrophic scars after being cured,this study by extracting the RNA of serum and biochip analysis,screening out the key genes of scar hyperplasia after burns,and bioinformatics analysis to study the long-chain non-coding RNA（Lnc RNA）expression profile changes in large areas of burn patients’ serum and normal human serum.And then this study screens out the key genes of scar hyperplasia for improving the prevention and treatment of post-burn hypertrophic scars,thus can provide new ideas and possible interventions.MethodIn this experiment,5 patients were selected as the observation group.These observation groups were patients who were diagnosed with burns greater than 30% of the body surface area and the depth of burns were deep II to III degrees in the First Affiliated Hospital of Anhui Medical University in 2019.The sampling time was 3 months after the wound surface healing.Another 5 normal samples were selected as the control group.These samples had no history of burns and scalds,and the results of physical examination were healthy.The physical examination method of the control group was to draw a 5ml venous blood sample on an empty stomach in the morning.The physical examination method of the patients in the observation group was to take a 5ml venous blood sample on an empty stomach in the morning that 3 months after the injury.In this experiment,RNA in serum was extracted after suitable conditions and operations.We label and hybridize the RNA.The expression levels of Lnc RNA in the serum of normal and post-burn scar hyperplasia patients were analyzed by biochip respectively and compare their fold change.A part of Lnc RNAs with large differences in expression were analyzed.Then do GO and pathway analysis.Results1.Compared with the Lnc RNA expression of the control group with the post-burn scar hyperplasia group,there were 2735 Lnc RNAs have more than 2 times difference（Fold change> 2.0,P<0.05）;Among them,there are 1626 Lnc RNAs that express an increase of more than 2 times,116 Lnc RNAs that are more than 5 times higher,and 11 Lnc RNAs that are more than 10 times higher.There are 1109 Lnc RNAs that express more than 2 times the downward adjustment,514 Lnc RNAs that are lowered by more than 5 times,and 246 Lnc RNAs that are lowered by more than 10 times.2.Compared with the m RNA expression of the control group with the post-burn scar hyperplasia group,there were 2166 m RNAs with a difference that more than 2 times（Fold change> 2.0,P<0.05）;Among them,there are 1395 m RNAs that express an increase of more than 2 times,79 m RNAs that are more than 5 times higher,and 12 m RNAs that are more than 10 times higher.There were 771 m RNAs expressions that were more than 2 times lower,323 m RNAs that were lowered by more than 5 times,and 140 that were lowered by more than 10 times.3.In the analysis of GO and Pathway,GO analysis showed that molecular function is related to protein binding and growth factor binding,cell components are related to plasma membrane part and cytoplasm,and biological processes are related to regulating cell response to various stimuli,signaling,etc.Pathway analysis shows that mapk and RAS signaling pathways and o-glycan biosynthesis are progressing.4.Differentially expressed Lnc RNAs are related to proteins FGF13,SMAD1,FIGF,MMP25.ConclusionBy comparing the serum of patients with post-burn scar hyperplasia with the serum of normal people,the expression levels of some specific Lnc RNAs changed significantly.Some Lnc RNAs can potentially regulate related genes.GO and Pathway analysis indicated that related Lnc RNAs play an important role in the pathogenesis and development of scar hyperplasia in patients with extensive burns,and provide directions for further selection of therapeutic anchors. |
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