Study On Processing Technology Of Gelsemium And Preliminary Investigation On Chemical Constituents

Objective:Gelsemium is Loganiaceae(Loganiaceae)gourd vine plant genus(Gelsemium)of the whole plant,with Qufeng attack drug Sanjie swelling,analgesic effect.Indications scabies scabies,eczema,scrofula,carbuncles,boils,bruises,rheumatism,neuralgia.Modern research shows that Gelsemium has anti-tumor,analgesic,enhance immunity,promote hematopoietic effects,especially for aspects of non-addictive analgesic and anti-tumor has obvious advantages,due to toxicity Gelsemium larger,and its therapeutic dose and toxic dose is very close,security index is small,difficult to control the dose,prone to toxic reactions are not rare clinical reports Gelsemium poisoning each year,limiting the widespread application of its clinical,preliminary studies have shown that after the discovery of Gelsemium by sand fried toxicities reduce,but also has a significant analgesic effect,to keep the efficiency attenuated the effect,but the impact of sand fry cooked Gelsemium many factors,resulting in unstable quality processed products.Therefore,the study of Gelsemium fried sand processing technology,stable and controllable low toxicity and efficient processing of Gelsemium sand fried Pieces for the further development of Gelsemium medicinal resources,provide a theoretical basis.Methods:The high-temperature heating method,analgesic efficacy in acute toxicity evaluation,on the basis of single factor test,select processing time,processing temperature as independent variables,the use of composite design(Central Composite Design)-Response Surface Methodology Gelsemium processing technology research.Based on the research of processing technology based on the use of UV spectrophotometric method to Gelsemium total alkaloids Index,optimization Gelsemium processed products of alcohol extraction process by the method of orthogonal experimental design;Finally,the alkaloid precipitation reagent identification methods,UV spectrophotometry and HPLC method,the chemical composition of Gelsemium concocted after a preliminary study.Results:Under Gelsemium 190-230℃ temperature after 10-60 minutes of processing,after processing by the attenuated significantly reduced,still retains some analgesic effect,the appearance of the processed products,toxicity and analgesic efficacy integrated consider,in the processing temperature of 200-220℃,processing time is preferably within 15-30min best technology,the best processing technology of Gelsemium,processing temperature of 210℃,processing time 15min.Gelsemium ethanol extract of processed products can be precipitation reaction with picric acid,potassium iodide,bismuth and silicon acid reagent;after all-wavelength ultraviolet scan found Gelsemium processed products and raw products full wavelength ultraviolet contour diagram similar fine not exactly the same structure,with a total alkaloid content of about 50%reduction;color using Elite column(250mm × 4.6 mm,5μm),acetonitrile-5mmol ammonium acetate-acetic acid(PH=3)gradient elution;detection wavelength:254 nm;flow rate:1.0 mL/min;column temperature:30℃;injection volume:10μL under chromatographic conditions.By high performance liquid chromatography analysis revealed significant changes in the chemical composition before and after processing,the proportion of the original chemical composition changes,resulting in the disappearance of new components and some chemical components.Optimized processing study Processed Products ethanol extract of Gelsemium found Gelsemium processed products after crushing,8 volumes of 95%ethanol extracted three times,the highest total alkaloid content;Conclusion:Preferred Gelsemium processing technology is stable,simple and feasible,predictive good,fully reflects the post-processing Gelsemium attenuated by the deposit effective role.Optimal alcohol extraction process is simple,low extraction cost,high total alkaloid content of the resulting benefits.Preliminary results show that the chemical composition before and after processing Gelsemium,significant changes in the chemical composition,may be concocted by Gelsemium "attenuated deposit effect" material basis.