Study For Expression Of Ribonucleotide Reductase M2 Subunit In Multiple Myeloma

Objective:multiple myeloma(MM)is a common malignancy in the hematologic system.It is characterized with abnormal proliferation of plasma cells and secretion of a large number of monoclonal immunoglobulin or its fragments.The incidence is second only to lymphoma in the hematologic malignancies.Transfer affects the ability of the size is one of the important characteristics that affect prognosis of malignant tumor,multiple myeloma cells prone to marrow infiltration and invade other organs,but the illness and happen to MM marrow infiltration mechanism is unclear.Ribonucleotide reductase is the rate-limiting enzyme in DNA synthesis,and is the only enzyme that can restore ribonucleotide to deoxyribonucleotide,which plays a key role in DNA synthesis and repair.RRM2,a small subunit of RR(RRM2),is a necessary group for the biological activity of RR,and also a key factor in determining RR activity.A large number of studies have shown that abnormal expression of RR is gastric cancer,breast cancer,colon cancer,and other important factors in the process of malignant tumours,its expression level associated with the ability to transfer of tumor,but at the moment about RRM2 express domestic has not been reported in multiple myeloma.This topic aims to study RRM2 expression in MM,analyze the relationship with the staging and prognosis,and may for MM inspection diagnosis,treatment monitoring and prognosis evaluation provides a new train of thought,of MM pathogenesis and therapeutic targets for the future provide experimental foundation and theoretical basis for further research.Methods:A total of 30 bone marrow samples were collected from 2 groups,of which 20 were in the MM group,all of which were patients with multiple myeloma diagnosed at the beginning of the clinical trial,and the myeloma cells were more than 10% in the bone marrow report.10 patients which were diagnosed with pure iron deficiency anemia(IDA)were in the control group.Thirty specimens were collected and grouped according to the case information.In addition,ISS was divided into groups according to the international staging system.Specimen collection immediately after extraction of bone marrow mononuclear cells by density gradient centrifugation,and hold-80 ℃.Bone marrow mononuclear cells in total RNA extracted by qiagen method,the concentration of total RNA extraction after test,test qualified samples and then to reverse transcription PCR,and then use the real-time fluorescent quantitative PCR(Realtime-PCR)detection of each specimen RRM2 m RNA expression level,the relative expression level(plus or minus s)said.SPSS17.0 software was used for statistical analysis.T test was used to analyze the differences in RRM2 expression in MM patients and control group samples.Variance analysis was used for RRM2 m RNA expression in patients with different stages.P<0.05 was considered statistically significant.Results: 1: the experimental group and control group two groups of samples RRM2 m RNA relative expression of the experimental results expressed in(±s),the MM relative expression of the experimental group(2.31±0.94),the control group(0.73±0.30),two groups of samples statistical differences in expression level between group(t = 6.897,P < 0.01),the expression level and the experimental group is significantly higher than the control group.2: international staging system ISS for grouping experimental samples,the results are: the three experimental groups ISS group 5 cases RRM2 m RNA Ⅰ period(1.44±0.54),ISS Ⅱ period set of 7 cases of RRM2 m RNA(2.11±0.57),the ISS Ⅲ phase of the group of 8 cases of RRM2 m RNA(3.03±0.88),three groups of experiments variance test samples,the MM of ISS staging in patients with three groups there were significant differences between the relative expression level(F = 8.188,P < 0.01),three contrast between two groups,the ISS stage Ⅲ with ISS Ⅰperiod and ISS Ⅱ group there were significant differences(t1 = 4.036,P < 0.01,t2 = 2.432,P< 0.05),while the ISS stage Ⅰ group and the ISS Ⅱ expression quantity no statistical difference between group(t =-2.043,P > 0.05).3.The relative expression of RRM2 m RNA in the experimental group was positively correlated with the proportion of myeloma cells in the patient’s bone marrow and the level of serum of the serum level of 2-mg(r1=0.666,r2=0.645,P<0.01).Conclusion:The expression of RRM2 m RNA in MM group was significantly higher than that in control group,indicating that RRM2 is up-regulated in MM.There was an increasing tendency of RRM2 m RNA expression in the three experimental groups of ISS with international staging system,and the relative expression level of ISSⅢphase group was significantly higher than that of ISSⅠand ISSⅡphase.The results showed that the expression of RRM2 increased with the increase of ISS staging.The expression level of RRM2 was correlated with the clinical stage and prognosis of MM,and could be used together with β2-MG to evaluate the prognosis and prognosis.Meanwhile,the relative expression of RRM2 m RNA in the experimental group was positively correlated with the proportion of original or immature myeloma cells and the level of β2-MG suggested by clinical information,suggesting that the expression of RRM2 is associated with the formation and development of myeloma cells in patients with MM And the secretion of β2-MG is closely related,which is of great significance for the further study of the metastasis and invasion ability of MM tumors,which can provide the basis for judging the prognosis and guiding the treatment.