| Objective1.Detection and comparison of intestinal flora structure between AD patients and healthy controls by high throughput sequencing.2.The clinical efficacy and safety of PTQX formulae for AD patients before and after intervention were compared,and the changes in intestinal flora were detected and analyzed,so as to preliminarily explore the effect of PTQX formulae on intestinal flora.Methods1.Clinical trial:a total of 60 subjects were included,including 30 patients with atopic dermatitis and 30 healthy controls.The intestinal flora questionnaire was filled out.30 cases of atopic dermatitis in patients with a total of 4 weeks treatment,observation of week 0,2 weeks,4 weeks treatment,patients with record analysis respectively on the point of IGA grade,SCORAD scores,EASI scores,POEM,DFI)score,CDLQI score,at 0,4 weeks fecal samples collected atopic dermatitis patients,healthy controls in the team to collect stool samples directly.2.Intestinal flora detection analysis:sending all collected stool samples to TaiChang gene technology co.LTD.Shanghai high-throughput sequencing lab for detection of bacteria in the intestine,will test data combined with clinical data,analysis of atopic dermatitis patients and healthy intestinal flora and contrast ridging call flora changes before and after the intervention,the preliminary exploration ridging blessed are side effects on intestinal flora.Results1.Comparison of intestinal flora between atopic dermatitis patients and healthy controls.(1)Baseline condition of subjects:all the 30 patients with atopic dermatitis and the 30 healthy controls included in this study were permanent residents in guangdong province.There was no significant difference in gender and age between the two groups(P>0.05).(2)Clinical characteristics:a total of 30 patients with AD were included in the study.In terms of dietary habits,vegetarian diet accounted for 20.00%in 6 patients,meat diet accounted for 23.33%in 7 patients,and balanced diet accounted for 56.67%in 17 patients.In terms of defecation,19 patients had defecation once a day,forming soft stools,accounting for 63.33%;4 patients had defecation once a day,and large feces were hard,accounting for 13.33%;6 patients had feces once every two days,and large feces were hard,accounting for 20.00%;1 patient had feces greater than once every two days,and large feces were hard,accounting for 3.33%.In terms of previous history,19 patients were associated with other allergic diseases,accounting for 63.33%.In terms of family history,17 patients had family history of allergic diseases,accounting for 56.67%.(3)Clinical curative effect:in 30 cases of AD patients before and after drug clinical index 0,2 weeks and 4 weeks EASI scores,SCORD score,DFI score changes not statistically difference(P>0.05),POEM score was statistically difference(P=0.024<0.05).In four weeks,compared with zero phase,EASI scores decreased from 4.87 to 3.15,SCORD score decreased from 28.61 to 20.63.IGA scores at 0,2 and 4 weeks were statistically significant(P=0.041<0.05).A total of 22 cases of AD patients were included between the ages of 5 and 16 years old.CDLQI score of the scale was performed at the time points of 0,2 and 4 weeks,and there was no statistical difference between the three groups(P=0.082>0.05).(4)Intestinal flora detection and analysis:A total of 90 qualified fecal samples were collected,including 60 in the atopic dermatitis patients group(before and after the intervention of TCM)and 30 in the healthy control group.The results showed that:①the Alpha diversity of the three groups of samples,the obserbed otus,chao and ace index of AD patients were slightly lower than that of the healthy control group,but the differences between the groups were not statistically significant(P>0.05).Shannon index:AD.OW group>AD.4W group>healthy control group;Simpson index:AD.OW group<AD.4W group<healthy control group,indicating that the diversity of intestinal flora of AD patients was higher than that of healthy people,but the difference was not statistically significant(P>0.05).The observed otus,chao and ace indexes of the intestinal flora observed by TCM observed the observed otus,chao and ace indexes increased,shannon index decreased in the fourth week,and Simpson index increased in the fourth week,but the differences were not statistically significant(P>0.05).②in terms of bacterial diversity,the relative abundance of Firmicutes in AD patients was higher than that of the healthy control(P>0.05),the relative abundance of Candidate_division_TM7 was higher than that of the healthy control(P<0.05),and the relative abundance of Bacteroidetes was lower than that of the healthy control(P>0.05).At the genus level,the relative abundance of Bacteroides was higher than that of the healthy control(P>0.05).The relative abundance of Phascolarctobacterium,Candidate_division_TM7_norank,Turicibacter,treptococcus,RF9_norank,Ralstonia,Caulobacteraceae_uncultured,Acidaminococcus,Caulobacte,Solobacterium is higher than the healthy control(P<0.05).Faecalibacterium,Raoultella,Butyricimonas and Stenotrophomonas have lower relative abundance than healthy controls(P<0.05).Before and after intervention;Comparison of Firmicutes before and after the intervention of PTQX formulae:at the phylum level of bacterial classification,the relative abundance of Firmicutes increased 4 weeks after the intervention of PTQX formulae,but the difference was not statistically significant(P>0.05),while the relative abundance of Bacteroidetes decreased,and the difference was statistically significant(P<0.05).On the level of bacterial classification and genus,the relative abundance of Bacteroides decreased after 4 weeks of intervention of PTQX formulae,but the difference was not statistically significant(P>0.05),while that of Faecalibacterium and Raoultella increased,and the difference was statistically significant(P<0.05).Conclusion1.There was no significant difference between the intestinal flora diversity index of patients with AD and the healthy controls,but the relative abundance of some of the flora was different between the two groups.At the phylum level,the relative abundance of Candidate_division_TM7 was higher than that of the healthy controls.At the genus-level,Phascolarctobacterium,Candidate_division_TM7_norank,Turicibacter,treptococcus,RF9_norank,Ralstonia,Caulobacteraceae_uncultured,Acidaminococcus,Caulobacter,Solobacterium have a higher relative abundance than the healthy control.Faecalibacterium,Raoultella,Butyricimonas and Stenotrophomonas have lower relative abundance than healthy controls.2.PTQX formulae has no obvious effect on the Alpha diversity index of intestinal bacteria in AD patients,but the relative abundance of Bacteroidetes can be reduced and that of Faecalibacterium and Raoultella can be increased. |
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