Effect Of ORP-150 Gene Overexpression On The Occurrence And Development Of Ovarian Cancer

Objective: This study was to investigate the expression of ORP-150 in ovarian cancer and non-ovarian cancer tissues,and to explore the relationship between ORP-150 abnormal expression and different types,differentiation of ovarian cancer,different clinical stages.In vitro experiments,we observed the effect on the phenotype of ovarian cancer cells by ORP-150 gene knockout,and to explore the possible molecular mechanism.Method:The expression of ORP-150 in ovarian cancer and ovarian normal tissues and benign tissues was compared by immunohistochemistry.The relationship between the expression of ORP-150 and the different pathological types,different clinical stages and grading of ovarian cancer was observed,the result was analyzed Non-parametric test analysis.The knockdown of ORP-150 was performed by si RNA interference technique.The knockdown efficiency was verified by q PCR and Western-blot.CCK-8 was used to detect the proliferation of ovarian cancer cells after knocking down ORP-150 gene.The effect of knockdown ORP-150 gene on the clonal ability of ovarian cancer cells was investigated by cloning assay.The effect of knockdown ORP-150 gene on the migration of ovarian cancer cells was studied by transwell and Wound Healingexperiments.The effect of ORP-150 gene on the cell cycle and apoptosis of ovarian cancer cells was detected by flow cytometry.The data were taken by single factor t test.Finally,the possible mechanism of ORP-150 gene knockdown on cell phenotype changes was investigated by Western-blot.Result: The expression of ORP-150 in ovarian normal tissues and benign tissues was lower than that in ovarian cancer(P<0.01).In patients with epithelial ovarian cancer,the expression of ORP-150 was higher in patients with stage III-IV than those in stage I-II(P <0.05).The patients with poorly differentiated patients had higher expression(P < 0.05),but there was no significant difference(P>0.05)between the various pathological types of ovarian cancer;The ORP-150 knockdown group could significantly inhibit the proliferation ability and clone formation ability of human ovarian cancer cell line SKOV-3(P<0.05);At the same time,compared with control group,the ability of transmembrane transfer and diffusion of ovarian cancer cells was decreased(P<0.05),but no significant difference in invasive ability in ORP-150 knockdown group in Wound Healing and transwell experiments;Finally,flow cytometry showed that there were more apoptotic cells in the experimental group,and the possible mechanism was further explored;It was found that the ORP-150 knockout group might affect cell repair and ultimately inhibit cell proliferation,and promote cell apoptosis through Wnt signaling pathway and Caspase Cascade.Conclusion:ORP-150 was highly expressed in epithelial ovarian cancer,and the lower the degree of pathological differentiation,the higher the clinical stage,the higher the expression level of ORP-150.The high expression of ORP-150 may promote the development of ovarian cancer by affecting Wnt signaling pathway and Caspase Cascade.