Effects Of IL-22 On Biological Behavior And Downstream Signaling Pathways Of MFC Cells

Objective: To study the expression of interleukin-22(IL-22)and its receptor IL-22 R(IL-22 R 1 and IL-10 R 2)in MFC cells of gastric cancer in mice and its effect on the biological behavior of MFC cells,and to explore the effect and mechanism of the activation of IL-22 on the downstream signaling pathway factors of MFC cells.Methods:(1)MFC cells of gastric cancer were cultured in mice.The levels of IL-22 and its receptor gene in MFC of gastric cancer cells were detected by q-PCR,and the expression differences of IL-22 and its receptor IL-22 R(IL-22 R 1 and IL-10 R 2)in MFC of gastric cancer cells were detected by western blot.(2)used in the process of gastric cancer cells in mice MFC to develop high(100ng/ml),low(1 ng/ml)of different concentrations of IL-22 intervention treatment,flow cytometry to detect gastric cancer cell apoptosis of MFC and trypan blue staining method to detect the survival rate of gastric cancer cells in MFC cells,IL-22 MTS method on gastric cancer cell activity of MFC,the influence of western blot detection IL-22 on gastric cancer cell in the MFC its downstream signaling pathways in the STAT-3,AKT,ERK gene expression and analyze its influence on gastric cancer cells in MFC.Results:(1)in MFC of gastric cancer cells,IL-22 and its receptor IL-22 R(IL-22 R 1 and IL-10 R 2)genes were both expressed.(2)after the intervention of IL-22 on MFC in gastric cancer cells,the number of MFC apoptosis in gastric cancer cells was reduced in both the high concentration group(100 ng/ml)and the low concentration group(1 ng/ml)compared with the blank control group,and the high concentration group was lower than the low concentration group.(3)after IL-22 intervention,there was no significant difference in cell activity and survival rate between the experimental group and the blank control group,nor was there any significant difference between the high-concentration group and thelow-concentration group.(4)after intervention with different concentrations of IL-22,western blot analysis showed that the phosphorylation of three proteins in the downstream of the signaling pathway,including STAT-3,AKT and ERK,were all up-regulated,and the high concentration group was higher than the low concentration group.Conclusion: IL-22 can promote the malignant development of tumor.Its mechanism may be to enhance the phosphorylation of downstream STAT-3,ERK and AKT signaling factors after binding to the receptor,thus inhibiting the apoptosis of cancer cells.