Evaluation Of Immune Function In Children With Mycoplasma Pneumonia

BackgroundMycoplasma pneumonia(MPP)is an acute inflammation of the lungs caused by mycoplasmapneumoniae(MP)infection.Mycoplasma pneumonia,an important pathogen of respiratory tract infection,is one of the more common infectious agents in childrens pneumonia,which can be transmitted through the air through oral and nasal secretions to cause respiratory system immune system damage.In recent years,the incidence of mycoplasma pneumonia in children has been increasing year by year,and the number of children seeking medical treatment for mycoplasmapneumoniae infection has been increasing.In addition to causing immune damage to respiratory system,it can also lead to other organdamage,there may even be a variety of extra-pulmonary manifestations associated with childhood asthma.Once children infected with mycoplasmal pneumonia,not only affect the quality of life of children,but also to the family spirit,economy caused multiple pressure.By detecting the activated molecules,immunoglobulin and some inflammatory response indexes of T cells in children,and analyzing their relationship with pulmonary function,this paper aims to provide help for the treatment and prognosis monitoring of the disease.ObjectiveTo observe the activation molecules,immunoglobulin and some inflammatory response indexes of T cells in children with mycoplasma pneumonia,and to analyze the relationship between them and pulmonary function indexes,in order to find out the clinical significance of detection indexes,and to provide some theoretical basis for MPP disease prevention and prognosis monitoring.Method1.Study Subjects:A total of 89 MPP patients in the Department of Pediatrics of the Affiliated Hospital of Vocational School were collected from June 2016 to May 2018.According to the diagnosis and the presence of wheezing,the patients were divided into non-wheezing group and wheezing group.There were 51 cases in non-wheezing group,including 27 males and 24 females,and 38 cases in wheezing group,including 19 males and 19 females.At the same time,32 healthy children,including 17 males and 15 females,were selected as the control group.All subjects had the right to informed consent,and there was no significant difference in age and sex.2.The test:Blood samples were collected from all subjects.The expression levels of CD3~+,CD3~+CD69~+,CD3~+CD25~+and CD3~+HLA-DR~+on T cell surface were detected by Flow cytometry,and c-reactive protein(CRP)was detected by immunofluorescence,the levels of IL-6,IL-10 and Ig E were detected by Elisa,Ig A,Ig M and Ig G were detected by immunoturbidimetry,and the pulmonary function index was detected by MPP,for example,Forced expiratory volume in one second,FEV1,and Forced vital capacity rate of one second,FEV1/FVC,Peak expiratory flow,PEF.The changes of CD3~+,CD3~+CD69~+,CD3~+CD25~+,CD3~+HLA-DR~+T cells and Serum Ig A,Ig M,Ig G,Ig E,IL-6,IL-10 and CRP were compared in each group.3.Statistical Processing:Using SPSS 17.0 software for statistical processing,data analysis before the test of normality and homogeneity of variance.Chi-square test was used for counting data,single-factor analysis of variance was used for measuring data conforming to normal distribution,independent sample t test was used for comparison between the two groups,mean±standard deviation((?)±s),Pearson method was used for correlation analysis,P<0.05 was statistically significant.Result1.Comparison of T cells between groups:The percentages of CD3~+T cell in the non-wheezing group and the wheezing group were significantly lower than that in the control group(P<0.001),and there was no significant difference between the non-wheezing group and the wheezing group.The percentages of CD3~+CD69~+,CD3~+CD25~+,and CD3~+HLA-DR~+T cellin the wheezing group were significantly higher than those in the control group and the non-wheezing group,while the percentage of CD3~+CD69~+T cell in the non-asthmatic group was significantly higher than that in the control group.The percentages of CD3~+CD25~+and CD3~+HLA-DR~+T cell in the non-wheezing group were not significantly different from those in the control group.2.Comparison of cytokines between groups:The serum levels of IL-6 and CRP in the non-wheezing group and wheezing group were significantly higher than those in the control group,and the serum IL-6 and CRP levels in the wheezing group were also higher than those in the non-wheezing group.Serum IL-10level in the wheezing group was significantly lower than those in the non-wheezing group and the control group,and there was no significant difference in IL-10 level between the non-wheezing group and the control group.3.Comparison of immunoglobulin molecules between groups:The content of Ig A,Ig G,Ig M and Ig E in wheezing group was higher than that in control group and non-wheezing group,but the content of Ig E in non-wheezing group was higher than that in control group.4.Comparison of pulmonary function indexes among different groups:Pulmonary function index FEV1/FVC and PEF in wheezing group were significantly lower than those in non-wheezing group,and FEV1 was not significantly different.5.Correlation analysis of pulmonary function index and detection index:The percentages of CD3~+,CD3~+CD69~+,CD3~+CD25~+,CD3~+HLA-DR~+T cell,and serum levels of Ig A,Ig M,Ig G,Ig E,IL-6,IL-10 were not significantly correlated with FEV1,FEV1/FVC,PEF,CRP was not correlated with FEV1,butnegatively correlated with FEV1/FVC and PEF(P<0.05).ConclusionChildren with MPP had different degree of activation of T cells,abnormal humoral immune function and decreased pulmonary function.The detection of activated T cells,CRP,IL-6 and Ig E has a certain guiding significance for MPP treatment monitoring and prognosis evaluation.