Effect Of Acupotomy On NGF/TRPV1 Expression And Visceral Hypersensitivity In Rats With Diarrhea Predominant Irritable Bowel Syndrome

Objective: To study the effects of acupotomy on the general condition,physical signs,diarrhea index,emotional state,TRPV1,NGF in colon tissue and TRPV1,NGF in spinal dorsal root ganglion of rats with diarrhea predominant irritable bowel syndrome.Objective to explore that Acupotomy can not only control the diarrhea symptoms of diarrhea type irritable bowel rats,but also influence its pathogenesis,so as to improve its visceral hypersensitivity.In order to prove the short-term and long-term advantages of acupotomy in the treatment of diarrhea predominant irritable bowel syndrome,and through the comparison of two acupoint selection schemes,more clinical thinking is given,hoping to provide some basis for the clinical application of acupotomy.Methods: 40 SD rats were randomly selected as a group after a week of adaptive feeding.The general condition,mental state and feces of all rats before modeling were recorded.Methods: the rats were exposed to seven different stress sources:(1)water shortage for 24 hours,(2)food deprivation for 24 hours,(3)painful tail clamping for 1 minute,(4)exposure to 45 ℃ for 5 minutes,(5)Swimming in water at 4 ℃ for 3 minutes,(6)inverted 12 hours /12 hours during the day and night,(7)horizontal vibration(120RPM)for 45 minutes.All stress programs were randomly applied every7 days for 3 weeks,in which no specific stress source was repeated for 2 consecutive days.On day 28,one week’s rest was followed by acute restraint stress,covering the shoulders,upper limbs,forelimbs and chest for one hour.The model was established by using relatively mild stress stimulation combined with peripheral sensitization,which improved the success rate of the model.On the7 th,14th,21 st and 28 th day of the experiment,the two treatment groups were intervened,the normal group(n = 6): normal feeding,no treatment,a total of 4 weeks;Model group(n = 6): modeling,no treatment,a total of 4 weeks;Acupotomy group 1(n = 6): the first treatment(day 7)was located in the bilateral articular process capsule of t10-l1 and L4-S1,with 0.1cm opening beside the spinous process and 2-3 transverse dissection;The second treatment(the 14 th day)was located at the left articular process capsule of t10-l1 and L4-S1,with 0.1cm opening between spinous processes and 0.4mm opening between spinous processes × After reaching the tough position,the needle was dredged longitudinally and stripped2-3 times horizontally;The third treatment(day 21)was located in the right articular process capsule of t10-l1 and L4-S1,with0.1 cm opening between spinous processes and 0.4 mm opening between spinous processes × After reaching the tough position,the needle was dredged longitudinally and stripped 2-3 times horizontally.The fourth treatment(28th day)was located in the right articular process capsule of t10-l1 and L4-S1,with 0.1cm opening between spinous processes and 0.4mm opening between spinous processes ×After reaching the tough position,the needle was dredged longitudinally and stripped 2-3 times horizontally.Acupotomy group 2(n = 6): for the first treatment,according to the "acupuncture point Atlas of experimental animals" formulated by the experimental acupuncture research association of Chinese acupuncture and Moxibustion Society,select "Zusanli" and "Dachangshu"(the fourth lumbar vertebrae,5mm apart,take both sides),and use 0.4mm × After reaching the deep fascia layer,the needle was peeled twice.Compression hemostasis was used to prevent infection after treatment.Open field test was carried out on the7 th and 28 th day of the experiment,and a series of indexes needed for diarrhea index were collected and measured on the 28 th day of the experiment.After the completion of acute stress restraint,the rats in each group were anesthetized and fixed with paraformaldehyde.The colon and spinal dorsal root ganglion tissues were removed and put into 4% paraformaldehyde fixative for standby.The average fluorescence intensity of TRPV1 and NGF in colon and spinal dorsal root ganglion was detected by immunofluorescence technique.Results:(1)after the intervention,the general condition and diarrhea index of the two treatment groups were significantly lower than those of the model group(P < 0.05)(2)Compared with the model group,the mood of the two treatment groups was significantly improved after the intervention(P < 0.05)(3)After intervention,there was no significant difference in emotional excitement,diarrhea index and the average fluorescence intensity of TRPV1 and NGF in colon tissue between the two groups(P > 0.05)(4)The average fluorescence intensity of TRPV1 and NGF in spinal dorsal root ganglion of acupotomology group 1 was higher than that of acupotomology group 2(P < 0.05).Conclusion: in the direction of acupotomy in the treatment of diarrhea predominant irritable bowel syndrome,the selection of acupoints in the spinal region is slightly better than that in Dachangshu and Zusanli,but the difference is not obvious,but it can affect the pathogenesis and improve abdominal diarrhea and visceral hypersensitivity by affecting TRPV1 and NGF in colon and spinal dorsal root ganglion.