Research On The Relationship Between Serum Vitamin A And T Lymphocyte Subsets In Children With Bronchial Asthma

ObjectiveTo study the correlation between serum vitamin A and CD4 + T cells,CD8 + T cells and CD4 + / CD8 + ratio in T lymphocyte subsets of children with bronchial asthma,so as to provide a scientific and reasonable basis for clinical diagnosis and treatment of asthma.MethodsFrom November 2019 to October 2020,73 children with asthma who were in the pediatric clinic and hospitalization of Shenyang Medical College Affiliated Central Hospital were selected as the asthma group,and 50 children with healthy physical examination were selected as the control group.High performance liquid chromatography and flow cytometry were used to determine serum vitamin A levels and T lymphocyte subsets of the two groups of children.Spss22.0 software was used for statistical analysis.Results1.Comparison of vitamin A levels between the asthma group and the control group: The vitamin A levels of the asthma group and the control group were(0.27±0.05)mg/L and(0.37±0.07)mg/L,respectively,and the difference was statistically significant(p<0.05)).2.Comparison of CD4+ T cells,CD8+ T cells,and CD4+/CD8+ levels between the asthma group and the control group: The CD4+ T cell levels of the asthma group and the control group were(49.64±6.25%)and(42.54±3.77%),respectively.P<0.05,the difference is statistically significant.The levels of CD8+ T cells in the asthma group and the control group were(31.62±4.11%)and(35.83±3.15%)respectively,P<0.05,the difference was statistically significant.The CD4+/CD8+ levels of the asthma group and the control group were(1.36±0.38)and(1.16±0.47)respectively,P<0.05,the difference was statistically significant.3.Comparison of the number of vitamin A deficiency cases between the asthma group and the control group: The number of vitamin A deficiency cases in the asthma group was higher than that in the control group,P<0.05,the difference was statistically significant.4.Comparison of CD4+ T cells,CD8+ T cells,and CD4+/CD8+ levels between children with normal vitamin A and children with vitamin A deficiency in the asthma group: CD4+ T cell levels between children with normal vitamin A and children with vitamin A deficiency Respectively(46.61±5.29%)and(51.03±6.21%),the difference between the two groups was statistically significant(P<0.05).The CD8+T cell levels of children with normal vitamin A and children with vitamin A deficiency were(33.37±3.26%)and(30.82±4.24%),respectively,and the difference between the two groups was statistically significant(P<0.05).The CD4+/CD8+ levels of children with normal vitamin A and children with vitamin A deficiency were(1.22±0.33)and(1.42±0.39),respectively,and the difference between the two groups was statistically significant(P<0.05).5.Pearson correlation analysis showed that there was a negative correlation between serum vitamin A and CD4+T cells and CD4+/CD8+ in children with asthma(r/p=-0.528/<0.05,r/p=-0.543/<0.05).There is a positive correlation between vitamin A and CD8+ T cells(r/p=0.502/<0.05).Conclusions1.The serum vitamin A level of children with asthma is lower than that of healthy children.Insufficient vitamin A levels may be one of the causes of asthma.2.The level of CD4+ T cells and the ratio of CD4+/CD8+ in children with asthma are higher than those of healthy children,and the level of CD8+ T cells is lower than that of healthy children.Abnormal cellular immune function may be one of the causes of asthma.3.In children with asthma,there are both vitamin A deficiency and normal vitamin A.The CD4+ T cell and CD4+/CD8+ levels of children with vitamin A deficiency are higher than those of children with normal vitamin A,and the level of CD8+ T cells is lower than those of children with normal vitamin A.4.In children with asthma,there was a negative correlation between Serum vitamin A levels and CD4+ T cells and CD4+/CD8+,and there was a positive correlation between vitamin A with CD8+ T cells,The lack of vitamin A can cause an imbalance in cellular immune function.