Correlation The Relationship Between Vaginal Microecology And Local Immunity And High-risk HPV Infection And Cervical Squamous Intraepithelial Lesions

Cervical cancer is a common cancer of female reproductive system.High-risk human papillomavirus(hrHPV)infection is closely related to its occurrence.The infection of hrHPVpersists can lead to cervical squamous intraepithelial lesions(SIL).If SILcan not be detected and treated in time,may frequently end to cervical cancer.Recent research has suggested that microbiota play an important part in humanp Hysiology,viral infections and diseases such as cancer.This study analyzed the relationship between vaginal microecology and hrHPV,microecology and different levels of SIL,as well as the concentrations of TNF-α,IL-6 in vaginal lavage fluid and different levels of SIL,to explore the impact of vaginal microecology on hrHPVinfection and progression of SIL,and the correlation between cervical local immune environment and SIL.Objective:To analyze the relationship between vaginal microecology and hrHPVinfection,different levels of SIL,and the relationship between vaginal local immune microenvironment and different levels of SIL,and to explore the influence of vaginal microecology on hrHPVinfection and the progression of SIL,as well as the relationship between local immune environment of cervical and the occurrence and progression of SIL.Try to propose a new way for clinical diagnosis and treatment on hrHPVinfection and cervical cancer.Methods:1.According to certain inclusion and exclusion criteria,238 hrHPVpositive patients were selected as the study group,and 468 hrHPVnegative women were selected as the control group.2.According to the same standard,patients with positive hrHPVwho need colposcopy were selected.The lavage fluid was collected before colposcopy,and the supernatant was frozen at-80℃after centrifugation.146 patients with cervical biopsy after colposcopy were divided into three groups according to the pathological results: nilm group: there was no atypical hyperplasia in histopathology;LSILgroup;HSILgroup.The results of vaginal microecology test among the three groups were analyzed and compared.28 cases were randomly selected from each group,TNF-α and IL-6 in vaginal lavage fluid was detected by ELISA.The concentrations of TNF-α and IL-6were compared among the three groups.3.Analyze the data by Spss25.0,Chi square test was used for count data,independent sample t test was used for measurement data in accordance with normal distribution,and one-way ANOVA test was used for multiple groups.Choice P<0.05 was statistically significant.Finding:1.There was no significant difference in the general clinical data between hrHPV-positive patients and hrHPV-negative women(P>0.05).The abnormal proportion of Lactobacillus,clue cells-positive,Gardnerella-positive,Nugent score,sialyl glycosidase-positive and coagulase-positive were correlated with hrHPVinfection(P<0.05),There was no correlation between other microecological indexes.2.There were 70 cases of non lesion(NILM group),39 cases of low-grade cervical squamous intraepithelial lesion(LSILgroup)and 37 cases of high-grade cervical squamous intraepithelial lesion(HSILgroup)in patients who have already had cervical biopsy.Gardnerella-positive was correlated with the grade of cervical lesions(P<0.05).3.The levels of TNF-α in vaginal lavage fluid of NILM group,LSILgroup and HSILgroup were respectively detection as 6.143±10.068pg/ml,2.578±3.870pg/ml and2.112±3.350pg/ml.It has really difference between NILM group and LSILgroup,NILM group and HSILgroup(P<0.05)There was no statistical significancebetween LSILgroup and HSILgroup(P>0.05).The concentrations of IL-6 in three groups are followed by7.965±3.897pg/ml,14.415±10.554pg/ml and 20.265±10.107pg/ml,and the differences were statistically significant(P<0.05).Further analysis showed that there were significant differences between each two group(P<0.05).Conclusion:1.Hr HPV infection is related to the proportion of Lactobacillus,bacterial vaginosis and Staphylococcus related aerobic vaginitis.The imbalance of Lactobacillus proportion,bacterial vaginosis and Staphylococcus related aerobic vaginitis may promote hrHPV infection.2.There is a correlation between cervical lesions and vaginal microecology.Bacterial vaginosis may be associated with the occurrence and development of SIL.3.The decrease of TNF-α in Local vagina is related to the occurrence and development of SIL,and the increase of IL-6 is related to the occurrence and development of SIL.The imbalance of local vaginal immune microenvironment may promote the occurrence and development of SIL.4.Adjusting the vaginal microecology and local immune microenvironment may provide a new idea for the control of hrHPV infection and the prevention and treatment of SIL.