| It is well known that bacterial infections are the major causes of chronic infection and death.Designing new antibacterial drugs with high-efficiency and low toxicity has become an urgent scientific problem.Metal-based nanozymes are widely used in the field of antibacterial therapy because of their excellent enzyme-like activity,easy preservation,easy preparation and low cost.However,the existing metal-based nanoenzymes need to use a lot of hydrogen peroxide.Therefore,it is of important research significance and application value to development metal-based nanozymes with high efficiency and broad-spectrum antibacterial properties without hydrogen peroxide.In this paper,Au@PtAg NRs,a multimetallic nanozyme with hollow structure,has been designed and synthesized using gold nanorods as template.The TEM images have been showed that Au@PtAg NRs maintain the rod-like structure of gold nanorods with hollow structure.The formation process of hollow structure was analyzed by ultraviolet absorption spectroscopy.X-ray photoelectron spectroscopy was used to determine that it has Pt,Au and Ag.Different oxidase substrates have used to prove that the hollow Au@PtAg NRs have oxidase-like and peroxidase-like activities,and can be further enhanced under acidic conditions.Subsequently,DPBF and TA were applied as active oxygen probes to verify that hollow Au@PtAg NRs can produce·O2-,H2O2and·OH.Taking Escherichia coli and Staphylococcus aureus as representatives of gram-negative and gram-positive bacteria,the antibacterial effect of hollow Au@PtAg NRs was explored.The minimum inhibitory concentration and minimum bactericidal concentration of nanozyme for Escherichia coli and Staphylococcus aureus are50μg/m L and 25μg/m L,respectively.It has been confirmed that they have spectral antibacterial properties and can change the permeability of bacteria.Finally,the antibacterial effect of hollow Au@PtAg NRs has also been verified in mice back wound infection model,and it has high biosafety. |
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