Inhibitory Effect Of Boschniakia Rossica Polysaccharides On Lipopolysaccharide-Induced Inflammatory Response In Mouse J774A.1 Macrophages

Objective : To investigate the inhibitory effect of Boschniakia rossica polysaccharides(BRPS)on the inflammatory response of J774A.1 macrophages induced by lipopolysaccharide(LPS).Methods: The inflammation model of macrophage was established by stimulating J774A.1 macrophage with LPS.The J774A.1 cells were randomly divided into the normal group,model group and BRPS groups(the concentrations of BRPS were 25,50 and 100mg/L,respectively).Cell viability was detected by cell counting kit-8,and the levels of interleukin-1β(IL-1β),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)were measured by the enzyme-linked immunosorbent assay.The protein expressions of cyclooxygenase-2(COX-2),toll-like receptor 4(TLR4),myeloid differentiation factor 88(My D88),interleukin-1 receptor-associated kinase 4(IRAK4),mitogenactivated protein kinase(MAPK),nuclear factor-κB(NF-кB)and IL-1β were determined with the western blot method.Result: 1.LPS had no obvious effect on the viabilities of J774A.1 macrophages in the concentration range of 100-2000ng/m L(P>0.05),but increased the secretion of TNF-α and IL-6(P<0.05),indicating the establishment of macrophage inflammation model.2.BRPS had no obvious effect on the viabilities of J774A.1 macrophages in the concentration range of 0-100mg/L(P>0.05).3.Compared with the normal group,the secretion of TNF-α,IL-6 and IL-1β in the J774A.1 macrophage inflammation model was significantly increased(P<0.05);compared with the model group,the secretion of TNF-α,IL-6 and IL-1β was reduced in the BRPS group(P<0.05).4.Compared with the normal group,the COX-2,TLR4,My D88,IRAK4,p-NF-кB,NF-кB,p-ERK,p-JNK,p-p38 protein levels in the model group were significantly increased(P<0.05);Compared with the model group,COX-2,TLR4,My D88,IRAK4,p-NF-кB,NF-кB,pERK,p-JNK,p-p38 protein levels were significantly reduced in the BRPS group(P<0.05).5.Compared with model group level,p-NF-кB,NF-кB and IL-1β protein expression were decreased in BRPS,BAY11-7082,and BAY11-7082+BRPS groups,(P<0.05);compared with BAY11-7082 group,the p-NF-кB and NF-кB levels were decreased in BAY11-7082+BRPS group(P<0.05).Compared with model group level,p-ERK and IL-1β protein expression were decreased in BRPS,U0126,and U0126+BRPS groups,(P<0.05);compared with U0126 group,the p-ERK levels was decreased in U0126+BRPS group(P<0.05).Compared with model group level,p-JNK and IL-1β protein expression were decreased in BRPS,SP600125,and SP600125+BRPS groups,(P<0.05);compared with SP600125 group,the p-JNK levels was decreased in SP600125+BRPS group(P<0.05).Compared with model group level,p-p38 and IL-1β protein expression were decreased in BRPS,SB203580,and SB203580+BRPS groups,(P<0.05);compared with SB203580 group,the p-p38 levels was decreased in SB203580+BRPS group(P<0.05).Conclusion: BRPS can inhibit the inflammatory response of J774A.1 macrophages induced by LPS,and its anti-inflammatory effect may be related to the inhibition of TLR4/NF-кB and MAPK signaling pathways.