Differentially Expressed MicroRNAs In Peripheral Blood Mononuclear Cells Of Non-segmental Vitiligo And Their Clinical Significance

Objective:To study the differentially expressed microRNA expression profile in peripheral blood mononuclear cells of non-segmental vitiligo,and try to screen molecular markers that could be used to evaluate the clinical stage of non-segmental vitiligo.Method:1.The miRNA expression profile in the peripheral blood mononuclear cells(PBMCs)of four patients with progressive non-segmental vitiligo(NSV)and four healthy controls(HC)was determined using high-throughput RNA sequencing.2.Through real time quantitative polymerase chain reaction(qRT-PCR),We verified the differentially expressed microRNAs in 26 patients with progressive NSV(P-NSV),26 patients with stable NSV(S-NSV)and 26 healthy controls.3.A miRNA-messenger RNA(m RNA)interaction network was created using bioinformatics tools.Results:1.High-throughput RNA sequencing technology sequencing results showed that there was a total of 323 differentially expressed microRNAs,of which 223 miRNAs were up-regulated and 100 miRNAs were down-regulated.This article summarized the top 10 up-regulated and 10 down-regulated differentially expressed miRNAs in PBMC of NSV patients.7 candidate miRNAs(hsa-miR-127-3p,hsa-miR-20a-5p,hsa-miR-142-5p,hsa-miR-340-5p,hsa-miR-101-3p,hsa-miR-335-5p,hsa-miR-668-3p)were relatively plentiful,| log2(Fold＿Change)|>2,P <0.001.2.The expression of hsa-miR-20a-5p,hsa-miR-335-5p,and hsa-miR-340-5p in PBMCs of progressive and stable NSV patients were significantly higher than those of healthy controls(P<0.001).In addition,compared with stable NSV patients,the expression of hsa-miR-20a-5p in PBMC of progressive NSV patients was also significantly higher.3.In the receiver characteristics curve analysis of has-miR-20a-5p for differentiating progressive and stable NSV patients from healthy controls in PBMCs,the area under curve(AUC)of the ROC curve was 0.92 and 0.81.Compared with patients in stable NSV,the hsa-miR-20a-5p was markedly increased in PBMCs of progressive NSV patients,and the AUC was 0.81.Conclusion:Divergently expressed miRNAs contribute to the pathogenesis of NSV and hsa-miR-20a-5p can be applied as a biosignature for stage assessment in PBMCs of patients with NSV.