Animal Study On Vascular A Ngiogenesis After Pulp Of Deciduous Teeth Implantation Into Dental Canal

Objective:Our study aims to explore the formation of early blood supply after the pulp of deciduous teeth implantation into the dental canal by the model of nude mice,and to provide theoretical basis for the in situ pulp regeneration in immature necrotic permanent teeth by the pulp of deciduous teeth transplantation.Methods:The extracted deciduous teeth were collected,and the pulp of deciduous teeth was extracted.According to the requirements of grouping,the pulp of deciduous teeth was treated with low temperature and implanted into the prepared orthodontic teeth,and then implanted subcutaneously into the nude mice.The experiment was divided into three groups: pulp of deciduous teeth + Matrigel group,pulp of deciduous teeth +Matrigel +VEGF group,and VITAPEX group.Eight weeks later,the nude mice were sacrificed,and the dental pulp blood supply was evaluated by histological and immunohistochemical methods.Results:1.HE staining showed that in the experimental group,the soft tissues rich in vascular structures filled most of the dental canal,while the hard tissue of the root canal wall did not change significantly.There was no soft tissue formation in the control group.2.Immunohistochemistry showed that human CD31 positive signal was distributed in the blood vessel wall in most of the samples of the experimental group,and mouse CD31 positive signal was distributed in all the samples.Human CD31 and mouse CD31 were negative in the control group.Conclusion:In the nude mice model,the dental pulp tissue implanted into the dental canal can establish the early blood supply between the dental pulp tissue and the surrounding tooth tissue.