Label-free Monitioring For Early Development Of Breast Ductal Carcinoma By Multiphoton Microscopy

Breast ductal carcinoma is one of the important branches of breast cancer,which seriously endangers the life and health of women.In the clinic,the early stages of ductal breast cancer face the risk of being overestimated or underestimated.Underestimation may cause patients to progress from early stage of breast ductal cancer to invasive ductal cancer,but overestimation may cause patients to over-treat.Therefore,improving the accuracy of early diagnosis of breast ductal cancer is a prerequisite for improving the success rate of breast-conserving surgery and the quality of life of patients with breast cancer.Over the past three decades,label-free multiphoton microscopy(MPM)have been widely used in biomedical imaging,with millimeter-level light sectioning capabilities,and advantages such as low phototoxicity,low photobleaching,and high-resolution label-free imaging.Firstly,label-free MPM was used to perform classification of ductal carcinoma in situ;secondly,we used label-free MPM to identify the early stages of breast ductal carcinoma,which include atypical ductal hyperplasia,ductal carcinoma in situ,and ductal carcinoma in situ with microinvasive;finally,we use label-free MPM to perform high-resolution imaging of macrophages and lymphocytes,and extract the multiphoton imaging features of macrophages and lymphocytes.In summary,label-free MPM can be used to monitor the early development of breast ductal carcinoma.With the continuous optimization of label-free MPM,label-free MPM is expected to become a powerful optical imaging tool for the early stage diagnosis and rapid intraoperative diagnosis of breast ductal carcinoma in the future.