Study On The Osteogenic Effects Of Degradation Microenvironment Of Magnesium-based Calcium Phosphorus Bioporous Active Scaffold

Purpose: In vitro and in vivo experimental studies are conducted to explore the osteogenic abilities,angiogenic capabilities and repair ability of bone defect of a new type of calcium-phosphorus coated magnesium based porous bioactive scaffold fabricated through template technology.Methods: In vitro experiments,mouse preosteoblasts(MC3T3-E1)and human umbilical vein endothelial cells(HUVECs)were used and cultured with the scaffold extract liquids.The cell viability of mouse pre-osteoblasts(MC3T3-E1)were tested to explore the biological safety and proliferation effects of the scaffold on pre-osteoblasts.Then,alkaline phosphatase expression detection,pre-osteoblast in vitro mineralization experiment and related RNA expression detection were performed on mouse pre-osteoblasts(MC3T3-E1)to explore the osteogenic promotion effects.The cell viability test of human umbilical vein endothelial cells(HUVECs)was carried out toexplore the biological safety and proliferation effects of the scaffold on endothelial cells.Then,the endothelial cell tube formation experiment was performed on the endothelial cells(HUVECs)to explore the angiogenic promotion effects.In vivo experiments,rats were used as experimental animals.The scaffold was implanted in the femoral condyle defect model of rats.The rats were sacrificed at 8 weeks,and histological observation,angiography and micro CT were performed to observe the growth of new bone and new blood vessels.The comparison between the two groups explore the osteogenesis and angiogenesis effects of the scaffold in the body.Results:The results of in vitro experiments showed that the extract liquids of the the porous magnesium-based calcium-phosphorus bioactive coated scaffold can promote cell growth and osteogenic differentiation and can also enhance mineralization and improve osteogenic related genes expression when cultured with mouse pre-osteoblast.While cultured with human umbilical vein endothelial cells,the results shows that the porous magnesium-based calcium-phosphorus bioactive coated scaffold can promote migration,proliferation and angiogenesis of HUVECs.In vivo,the porous magnesium-based calcium-phosphorus bioactive coated scaffold can effectively stimulates angiogenesis,osteogenesis and perfectly repairs the bone defect in the rat models as the porous magnesium-based calcium-phosphorus bioactive scaffold degraded.Conclusion: This study shows that the scaffold has excellent osteogenesis and angiogenesis promotion effects in vitro experiments,and has excellent ability to repair bone defects in vivo experiments.