| Objective:To explore the experimental conditions and histological changes of rabbit ear hypertrophic scar models established by multifunctional ion surgery.Methods:1.Divided twenty-four New Zealand large ear white rabbits randomly and equally into four groups,and marked the ventral flank of each rabbit ear with two circular surgical zones arranged up and down with a diameter of 1 cm.The fixed multifunctional electric ion surgical treatment machine voltage was 12 V.After local infiltration anesthesia,the metal contact was used to cauterize from the periphery of the marked area to the center for many times.In group A,the layer of the burn was superficial cartilage;in group B,the layer of the burn was from the cartilage layer to the cartilage membrane layer and the cartilage membrane was completely vaporized;in group C,the layer of the burn was deep dermis;the group D did not use multifunctional ion therapy and is removed with a scalpel full-thickness skin and remove the perichondrium.2.Observed the general changes of the wound healing of rabbit ears,recorded the time of complete epithelization,measured the thickness of the central scar with vernier caliper every 10 days from the 30 th day after operation,and calculated the scar hypertrophic index;the specimens were cut on 60 th postoperative day for pathological analysis(hematoxylin and eosin,Masson,and Van Gieson staining were included)and immunoblotting for CD31 and α-smooth muscle actin(α-SMA)expression levels..Results:1.The average epithelization time of wound in group D was the shortest,and the time in other groups was gradually prolonged with the deepening of wound level.The success rate of hypertrophic scar modeling in group B and group D was the highest,and the difference was not statistically significant(P=0.748).The scar hyperplasia index of group B and group D was significantly higher than that of group A and group C(P<0.05).The data of group B was significantly lower than that of group D at 30 days after operation(P<0.001).There was no significant difference between the two groups at 40days(P=0.086),and the data of the two groups showed a downward trend.2.Histological examination showed thickening of dermal spinous layer and granular cell layer,moderate abnormal structure,more disordered collagen fibers,accompanied by infiltration of inflammatory cells and capillary neovascularization in group B and group D,especially in group B,which was similar to the histopathological changes of human hypertrophic scar.In group A,there were severe structural abnormalities and severe cell degeneration and necrosis.In group C,collagen was arranged orderly and tightly without obvious inflammatory cell infiltration,similar to human physiological scar.α-SMA was not significantly different between groups B and D(P=0.13),and the expression of CD31 was lower in group B than in group D(P < 0.05).The content of both indicators in the A A and C group was lower than that in the previous two groups,and that in group A was lower than that in group C.Conclusion:The hypertrophic scar model of rabbit ear can be made by cauterizing to the cartilage membrane layer through burning multiple points layer by layer until the perichondrium is completely vaporized by using the multifunctional electric ion surgical treatment instrument under the voltage of 12 V,which is similar to the result of hypertrophic scar model made by excising the full-thickness skin and cartilage membrane layer. |
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