MiR-29b-3p Targets PIK3R1 To Promote The Proliferation And Migration Of Pc9 Cells Through The PI3K/AKT Pathway

Lung cancer is one of the malignant tumors with the highest incidence of cancer.Related reports and studies have found that smoking,air pollution,occupational exposure to inhalation of dust or harmful substances,genetic factors,radiation factors and dietary factors all easily lead to a rapid increase in the incidence of lung cancer.The histopathological grading of lung cancer determines that about 80-85% of lung cancers are non-small cell lung cancer.Although the diagnosis and staging of lung cancer have become more precise,the treatment of lung cancer has become more mature.However,the 5-year survival rate of NSCLC patients is still less than 20%.In the past ten years,many molecular targets for specific cancer subtypes in a variety of human malignancies have been successfully studied,such as epidermal growth factor(EGFR)mutant small molecule tyrosine kinase inhibitors(TKIs)application.However,TKIs resistance also inevitably appeared.Therefore,we still need to further understand the complexity of lung cancer genesis,so as to discover new diagnostic and therapeutic targets to provide more possibilities for lung cancer treatment.Micro RNA(miRNA)is a class of single-stranded non-coding RNA molecules containing 21-23 nucleotides.It mainly regulates the expression of mRNA of target genes through translation inhibition or messenger degradation.The current research on miRNA has discovered many important molecular mechanisms,which are closely related to cell life activities such as differentiation,proliferation,apoptosis,death,and carbohydrate and fat metabolism.Relevant studies have shown that although some miRNAs can promote the resistance of lung cancer cells to treatment,they can also be used as promising therapeutic targets to enhance the sensitivity of lung cancer cells to treatment.The phosphoinositide 3-kinase(PI3K)pathway plays a central role in growth factor signaling and tumorigenesis.PIK3R1 is the p85 regulatory subunit of PI3 K.The main function of the p85 subunit is to bind,stabilize and inhibit the p110 catalytic subunit until the receptor tyrosine kinase(RTK)is activated.The PI3K/AKT pathway plays a key role in both insulin and growth factors in cell metabolism and cell growth.The activation of the insulin-PI3 K cascade may promote carcinogenic transformation.Genetic changes in several proteins in the PI3 K signaling pathway have been observed in cancer-including p85,p110α,PTEN and AKT.Among them,PIK3R1 is dysregulated in many forms of cancer,including breast cancer,colon cancer and liver cancer.The purpose of this research is to explore the effect of miRNAs targeting PIK3R1 in non-small cell lung cancer on cell proliferation and migration and related mechanism research.Through a series of bioinformatics analysis and literature research to clarify the research direction,we have found the goal Protein PIK3R1 and miRNAs targeting PIK3R1.We changed the expression levels of miRNAs and PIK3R1 in pc9 cells to observe the changes in cell functions and related molecular mechanisms.The observed result is that miR-29b-3p promotes the proliferation and migration of non-small cell lung cancer cells by targeting PIK3R1,and miR-29b-3p also activates the PI3K/AKT pathway by targeting PIK3R1.We hope to explore this phenomenon to provide a reference for future research on miR-29b-3p and lung cancer resistance.