Effect Of Bladder Under High Pressure On The Expression Of NF-κB P65、TNF-α And ColⅢ In Rat Bladder Mucosa

Purpose: Bladder under high pressure can cause a series of pathological changes.We investigated the effect of bladder pressure on the expression of NF-κB 、 Col III and TNF-α in rat bladder smooth muscle cells.Methods: 28 healthy female SD rats were randomly divided into four groups,seven rats in each group:control group in 1 week,half obstruction in 1 week,control in 4 weeks and half obstruction in 4 weeks based on the half bladder outlet obstruction in rats model.After the experiment,we detected of bladder filling pressure by removing bladder specimens and tested the rat bladder leakage point pressure,bladder capacity and the index change of NF-κB P65、Col III、TNF-α though immunohistochemical technique in specimens.Result: The experimental results showed that the filling bladder pressure of rats with semi-obstruction for 1 and 4 weeks were significantly higher than that of the control groups.With the time prolongation,the filling bladder pressure was higher while the bladder capacity was larger within four weeks.The difference between the experimental groups and the control groups was statistically significant(P< 0.05).Compared with the control groups,NF-κB P65 、 Col III and TNF-α in the bladder epithelium of the experimental groups were significantly increased.As the half-obstruction time increased(but less than 4 weeks)the NF-κB P65、Col III and TNF-α expression was higher.Statistically,the differences between the two groups are significant.(P < 0.05).Conclusion: Bladder under chronic high pressure can lead to an increase of bladder filling pressure and an increase in functional bladder capacity.In this case,the expressions of NF-κB P65,Col III and TNF-α were significantly increased with the higher pressure.Therefore,NF-κB signaling pathway may prove to be an important signaling pathway of high stress and inducement of bladder fibrosis.